Measurement of urinary concentrations of the mycotoxins zearalenone and sterigmatocystin as biomarkers of exposure in mares.
Measurement of urinary concentrations of the mycotoxins zearalenone and sterigmatocystin as biomarkers of exposure in mares.
复制标题
测量尿液中霉菌毒素玉米赤霉烯酮和杂色曲霉素的浓度作为母马暴露的生物标志物。
DOI：
10.1111/rda.13054
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Fink-Gremmels J.
中科院分区：
文献类型：
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作者：
Takagi M;Uno S;Kokushi E;Sato F;Wijayagunawardane MPB;Fink-Gremmels J.
霉菌毒素可能影响动物健康，包括生殖。关于马暴露于受污染饲料的临床相关性知之甚少。本研究旨在（i）监测日本纯种马尿液样本中真菌毒素玉米赤霉烯酮（ZEN）及其代谢产物α-和β-玉米赤霉烯醇（α-和β-ZOL）和杂色曲霉素（STC）的水平，以及（ii）将这些发现与育种马生殖功效的潜在影响联系起来。使用了来自59个养殖场的63份尿样。当每匹母马被送到种马站时，从每匹母马中收集尿样和生殖记录。使用液相色谱-串联质谱法（LC-MS/MS）测量ZEN、α-和β-ZOL以及STC的尿液浓度。ZEN、α-和β-ZOL在所有受检马的尿液中均可测量，表明ZEN在马饲料中的患病率。在63份样本中的7份中，还检测到STC，其水平范围为1.3至18.0 pg/mg肌酐。未观察到真菌毒素浓度与妊娠状态之间存在显著相关性。总之，尿液样本中真菌毒素的测量是监测尿液对多种真菌毒素的全身暴露的一种有用的非侵入性方法。
ContentsMycotoxins may affect animal health, including reproduction. Little is known about the clinical relevance of exposure of horses to contaminated feed. This study aimed at (i) monitoring the levels of the mycotoxins zearalenone (ZEN), with its metabolites α‐ and β‐zearalenol (α‐ and β‐ZOL), and sterigmatocystin (STC) in urine samples from thoroughbred mares in Japan and (ii) relating these findings to the potential effects on reproductive efficacy of breeding mares. Sixty‐three urine samples of breeding mares from 59 breeding farms were used. Urine samples and reproductive records were collected from each mare when it was presented to the stallion station. Urinary concentrations of ZEN, α‐ and β‐ZOL, and STC were measured using liquid chromatography‐tandem mass spectrometry (LC‐MS/MS). ZEN, α‐ and β‐ZOL were measurable in the urine of all examined mares, indicating the prevalence of ZEN in equine feeds. In seven of the 63 samples, STC was also detected at levels ranging from 1.3 to 18.0 pg/mg creatinine. No significant correlation between the concentrations of mycotoxins and pregnancy status was observed. In conclusion, measurement of mycotoxins in urine samples is a useful non‐invasive method for monitoring the systemic exposure of mares to multiple mycotoxins.