Palladium film decoupler for amperometric detection in electrophoresis chips
Palladium film decoupler for amperometric detection in electrophoresis chips
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DOI：
10.1021/ac000452u
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发表时间：
2001-02-15
影响因子：
7.4
通讯作者：
Chen, CH
中科院分区：
文献类型：
--
作者：
Chen, DC;Hsu, FL;Chen, CH
本文证明了钯金属膜可用于将电泳芯片中的电化学检测电路与电泳分离电路解耦。 Pd 固态场解耦器以及工作电极被热蒸发到塑料芯片上，并垂直穿过分离通道。样品区流过 Pd 解耦器后，在下游路径的工作电极处测量它们的电化学响应。由于电极位于分离通道上，因此电极通道对准不再是问题。对于 10 mM 磷酸盐 (pH 5.1) 中宽度约为 200 µm、深度约为 75 µm 的分离通道，在 570 V/cm 电场下，工作电极的噪声水平 < 15 pA。
Demonstrated in this article is that a palladium metal film can be applied to decouple the electric circuitry of electrochemical detection from that of the electrophoretic separation in an electrophoresis chip. The Pd solid-state field decoupler, as well as the working electrodes, is thermally evaporated onto the plastic chip and oriented vertically across the separation channel. After the sample zones flow over the Pd decoupler, their electrochemical response is measured at working electrodes in the downstream pathway. Because the electrodes are on the separation channel, the electrode channel alignment is no longer a problem. For a separation channel of roughly 200 mum in width and 75 mum in depth in 10 mM phosphate (pH 5.1), the noise level at the working electrode is < 15 pA at an electric field of 570 V/cm.