Expression of Placenta growth factor (PlGF) in non-small cell lung cancer (NSCLC) and the clinical and prognostic significance.
Expression of Placenta growth factor (PlGF) in non-small cell lung cancer (NSCLC) and the clinical and prognostic significance.
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DOI：
10.1186/1477-7819-3-68
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发表时间：
2005-10-13
影响因子：
3.2
通讯作者：
Jiang WG
中科院分区：
文献类型：
--
作者：
Zhang L;Chen J;Ke Y;Mansel RE;Jiang WG
胎盘生长因子(PlGF)是血管内皮生长因子(VEGF)家族的成员。已知PlGF的过度表达与病理性血管生成有关。本研究检测了PlGF在非小细胞肺癌(NSCLC)中的蛋白和消息水平的表达，目前还没有关于PlGF表达意义的报道。我们用免疫组织化学方法检测了91例非小细胞肺癌患者(n=91)中PlGF蛋白的表达及其与微血管密度(MVD)和临床预后的关系。此外，我们还应用SYBR Green化学实时定量聚合酶链式反应技术检测了正常肺组织和非小细胞肺癌肿瘤组织中PlGF基因的表达。肺癌细胞内PlGF阳性染色主要位于胞浆。38.5%的非小细胞肺癌患者有PlGF高表达。42.9%的非小细胞肺癌组织中存在高水平的MVD。高表达和低表达的肿瘤微血管密度分别为26.69vs.20.79，P=0.003。通过单因素和多因素分析，发现PlGF是一个独立的预后因素。实时荧光定量聚合酶链式反应结果显示，癌组织中PlGF mRNA的表达水平显著高于正常组织(P<0.005)，且III~IV期患者的PlGF mRNA水平显著高于I~II期患者(P=0.011)。非小细胞肺癌组织中PlGF的表达明显高于正常组织。它与微血管密度呈显著正相关，是非小细胞肺癌患者的一个独立因素。PlGF可能在非小细胞肺癌的发生发展和疾病进展中起着关键作用。
Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family. Over-expression of PlGF is known to be associated with pathological angiogenesis. This study examined PlGF expression at protein and message levels in non-small cell lung cancer (NSCLC), in which no reports on the significance of PlGF expression is available to date. We used immunohistochemistry to assess the PlGF protein and correlated PlGF with microvessel density (MVD), as well as clinical outcome in patients with NSCLC tumours (n = 91). In addition, we applied a real time quantitative PCR assay using SYBR Green chemistry to measure PlGF mRNA in normal lung tissues and NSCLC tumours. PlGF was positively stained mainly in cytoplasm of lung cancer cells. High level staining of PlGF was found in 38.5% NSCLC patients. A high level of MVD in NSCLC was found in 42.9% of cases. Tumours with high level and low level PlGF staining had a significantly different MVD (26.69 vs. 20.79, respectively, p = 0.003). Using both univariate and multivariate analyses, PlGF was found to be an independent prognostic factor. Real time PCR analysis revealed that PlGF mRNA was higher in the cancer tissue than normal tissue (0.95 ± 0.19 vs. 0.57 ± 0.24; p < 0.005) and that PlGF mRNA was significant higher in III-IV stage patients than in I-II stage patients (1.03 ± 0.20 vs. 0.80 ± 0.17; p = 0.011). PlGF expression is significantly more in NSCLC tumour tissues than in matched normal tissues. It has a significant positive association with MVD and is an independent factor for NSCLC patients. PlGF may have a pivotal role in NSCLC development and disease progression.