SEQUENTIAL STUDY OF LESION DEVELOPMENT IN EXPERIMENTAL HAEMOPHILUS PLEUROPNEUMONIA
SEQUENTIAL STUDY OF LESION DEVELOPMENT IN EXPERIMENTAL HAEMOPHILUS PLEUROPNEUMONIA
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DOI：
10.1016/s0034-5288(18)30687-8
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发表时间：
1987-03-01
影响因子：
2.4
通讯作者：
FARRELL, RL
中科院分区：
文献类型：
--
作者：
LIGGETT, AD;HARRISON, LR;FARRELL, RL
最近断奶的猪被胸膜肺炎嗜血杆菌（放线杆菌）血清型5需氧性感染。在暴露于血友病菌后3、6、12和18小时以及1、2、4和7天，处死一对动物并进行尸检。在感染后3小时和6小时观察到肺水肿伴多灶性点状出血和弥漫性嗜中性细支气管炎和肺泡炎。在感染后12和18小时，凝固性坏死的局灶性区域在强烈化脓的区域中发展。在感染后1天和2天，坏死区域被变性白细胞的致密带包围，并含有该疾病特征的无法识别的圆形和细长细胞。在亚急性病变中，脓肿外缘周围形成一层厚厚的肉芽组织。肺泡渗出液中的大多数圆形和细长的细胞不能通过酶组织化学或电镜检查来识别。中性粒细胞在不同阶段的退化和恶化提供了强有力的证据，一些细胞是这种类型。这些发现表明，中性粒细胞可能在病变的发展中发挥早期和重要的作用。
Recently weaned pigs were infected aerogenically with Haemophilus (Actinobacillus) pleuropneumoniae, serotype 5. At three, six, 12, and 18 hours and one, two, four and seven days after exposure to haemophili a pair of animals were killed and necropsied. Pulmonary oedema with multifocal petechial haemorrhages and a diffuse neutrophilic bronchiolitis and alveolitis were observed at three and six hours after infection. Focal areas of coagulative necrosis developed in areas of intense suppuration at 12 and 18 hours after infection. At one and two days after infection, necrotic areas were surrounded by dense bands of degenerating leucocytes and contained unidentifiable round and elongated cells characteristic of this disease. In subacute lesions a thick layer of granulation tissue formed around the outer margin of developing abscesses. Most of the round and elongated cells in alveolar exudates could not be identified by enzyme histochemistry or electron microscopic examination. Neutrophils in various stages of degeneration and deterioration provided strong evidence that some of the cells were of this type. These findings suggest that neutrophils may play an early and significant role in development of lesions.