THE APPEARANCE, DENSITY, AND DISTRIBUTION OF MERKEL CELLS IN HUMAN EMBRYONIC AND FETAL SKIN - THEIR RELATION TO SWEAT GLAND AND HAIR FOLLICLE DEVELOPMENT
THE APPEARANCE, DENSITY, AND DISTRIBUTION OF MERKEL CELLS IN HUMAN EMBRYONIC AND FETAL SKIN - THEIR RELATION TO SWEAT GLAND AND HAIR FOLLICLE DEVELOPMENT
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DOI：
10.1111/1523-1747.ep12665903
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发表时间：
1995-03-01
影响因子：
6.5
通讯作者：
HOLBROOK, KA
中科院分区：
文献类型：
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作者：
KIM, DK;HOLBROOK, KA
采用免疫标记技术对乙二胺四乙酸分离获得的表皮和真皮片研究了人胚胎和胎儿皮肤中默克尔细胞的密度和分布。默克尔细胞通过已知的细胞角蛋白标记物 CK20 和 CK18 进行鉴定。 Merkel 细胞早在估计胎龄 (EGA) 56 天时就在手掌表皮中表现出 CK20 免疫反应性 (133.11 +/- 44.27 个细胞/mm(2))。密度迅速增加，在 80-90 dEGA 时达到最大值超过 1400 个细胞/mm(2)。在这个阶段，细胞沿着初级表皮脊分布。在EGA 100 d以上胎儿的掌表皮中，Merkel细胞的分布也呈现出相同的模式，但密度随后逐渐降低。在小汗腺的导管和腺体部分中没有观察到默克尔细胞。在 EGA 75 d 的胎儿中，在毛状皮肤的表皮层中首次发现了一些细胞。 100 d EGA时,仅观察到少量Merkel细胞,主要分布在毛桩和球状毛桩中;在较大胎儿中,Merkel细胞在毛囊漏斗部和隆起处分别呈环状排列和聚集体;Merkel细胞形态呈球状和树突状;树突状细胞与球状细胞的比例逐渐增加,直至无毛和毛状皮肤中Merkel细胞密度最高的时期;位于真皮层的Ah Merkel细胞呈球状，根据所得结果，我们推测Merkel细胞可能在皮肤发育中小汗腺和毛囊原基的形成和增殖中发挥一定的功能作用。
The density and distribution of Merkel cells in human embryonic and fetal skin were studied using an immunolabeling technique on epidermal and dermal sheets obtained by ethylenediamine tetraacetic acid separation. Merkel cells were identified by the known cytokeratin markers CK20 and CK18. Merkel cells showed CK20 immunoreactivity as early as 56 d estimated gestational age (EGA) in the palmar epidermis (133.11 +/- 44.27 cells/mm(2)). The density increased rapidly, reaching a maximum of more than 1400 cells/mm(2) at 80-90 dEGA. At this stage, the cells became distributed along the primary epidermal ridges. In the palmar epidermis of fetuses older than 100 d EGA, the distribution of Merkel cells showed the same pattern, but the density then decreased gradually. Merkel cells were not observed in ductal and glandular portions of eccrine sweat glands. In the epidermal sheets of hairy skin, a few cells were first seen in the fetus at 75 d EGA. At 100 d EGA, only a few Merkel cells were observed, mostly in the hair pegs and bulbous hair pegs, In the older fetus, ringlike arrangements and aggregates of Merkel cells were prominent in the infundibulum and bulge of hair follicles, respectively, Merkel cells were both globular and dendritic in shape, The ratio of dendritic to globular cells increased gradually until the period of highest Merkel cell density in both the glabrous and hairy skin, Ah Merkel cells located in the dermis were globular in shape, In accord with the results obtained, we postulate that Merkel cells may have some functional role in the formation and proliferation of eccrine sweat glands and hair follicle anlagen in developing skin.