The effects of osmotic stress on the cell wall-plasma membrane domains of the unicellular streptophyte, Penium margaritaceum
The effects of osmotic stress on the cell wall-plasma membrane domains of the unicellular streptophyte, Penium margaritaceum
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DOI：
10.1007/s00709-021-01644-y
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发表时间：
2021-04-30
期刊：
影响因子：
2.9
通讯作者：
Palacio-Lopez，Kattia
中科院分区：
文献类型：
--
作者：
Domozych，David S.;Kozel，Li;Palacio-Lopez，Kattia
MargaritaceumPenum margaritaceum是一种单细胞合生植物(基本链藻或轮藻)，已被用作研究链霉菌细胞壁和阐明在陆地植物进化中关键的生物适应的模式生物。当Peniumum在山梨醇增强型培养基(即高渗介质)中孵育时，1000-1500条赫氏链在几分钟内形成，并将质膜连接到细胞壁。随着时间的推移，细胞适应了这种渗透胁迫，细胞壁和质膜区域发生了进一步的显著变化。外层细胞壁层的同型半乳糖原点阵显著减少，并伴随着高度细长的“丝状”表型的形成。在CW和质膜之间出现明显的周边增厚，并含有膜性成分和分枝的颗粒状基质。对这些增厚的单抗标记表明存在鼠李半乳尿苷-I表位。驯化还导致细胞的空泡网络和大型自噬的增殖。Penium对渗透胁迫的驯化能力提供了古代合生植物从水生到陆地生存的转变的洞察。
Penium margaritaceumis a unicellular zygnematophyte (basal Streptophyteor Charophyte) that has been used as a model organism for the study of cell walls of Streptophytes and for elucidating organismal adaptations that were key in the evolution of land plants.. WhenPeniumis incubated in sorbitol-enhance medium, i.e., hyperosmotic medium, 1000–1500 Hechtian strands form within minutes and connect the plasma membrane to the cell wall. As cells acclimate to this osmotic stress over time, further significant changes occur at the cell wall and plasma membrane domains. The homogalacturonan lattice of the outer cell wall layer is significantly reduced and is accompanied by the formation of a highly elongate, “filamentous” phenotype. Distinct peripheral thickenings appear between the CW and plasma membrane and contain membranous components and a branched granular matrix. Monoclonal antibody labeling of these thickenings indicates the presence of rhamnogalacturonan-I epitopes. Acclimatization also results in the proliferation of the cell’s vacuolar networks and macroautophagy.Penium’s ability to acclimatize to osmotic stress offers insight into the transition of ancient zygnematophytes from an aquatic to terrestrial existence.