Corticosteroids enhance hepatic injury following ischemia-reperfusion.
Corticosteroids enhance hepatic injury following ischemia-reperfusion.
复制标题
皮质类固醇会加重缺血再灌注后的肝损伤。
DOI：
10.1016/s0041-1345(01)02514-3
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发表时间：
2001
影响因子：
0.9
通讯作者：
L. Cicalese
中科院分区：
文献类型：
--
作者：
P. Sileri;S. Schena;J. Fukada;C. Rastellini;J. Pirenne;E. Benedetti;L. Cicalese
材料和方法在本研究中使用体重200 - 250 g的雄性ACI大鼠（Sprague-Dawley，Inc，Indianapolis，Ind）。将动物随机分为两组。在部分肝缺血研究前1小时，用2 mg/kg静脉内（IV）甲泼尼龙处理动物，而对照动物接受相同体积的IV盐水溶液。在每组（n 4）中，通过夹闭肝左外叶和中叶的门静脉和动脉分支60分钟来进行部分肝缺血。再灌注后2、6、24和48小时处死动物。测定血清AST、ALT和LDH，获得肝脏样品并检查形态学、DNA片段化（DNA梯状）和细胞凋亡评价（TUNEL）。使用Student t检验和Mann-Whitney U检验进行统计分析。
MATERIALS AND METHODSMale ACI rats (Sprague-Dawley, Inc, Indianapolis, Ind), weighing 200 to 250 g, were used in this study. Animals were randomly divided into two groups. One hour prior to partial liver ischemia study animals were treated with 2 mg/kg intravenously (IV) of methylprednisolone, whereas control animals received the same volume of IV saline solution. In each group (n 4), partial liver ischemia was done by clamping the portal and arterial branches to the left lateral and median lobes of the liver for 60 minutes. Animals were killed at 2, 6, 24, and 48 hours after reperfusion. Serum AST, ALT, and LDH were measured and liver samples were obtained and examined for morphology, DNA fragmentation (DNA laddering), and evaluation of apoptosis (TUNEL). Statistical analyses were performed using Student’s t test and the Mann–Whitney U test.