Oxidation-resistant membrane phospholipids can explain longevity differences among the longest-living rodents and similarly-sized mice
Oxidation-resistant membrane phospholipids can explain longevity differences among the longest-living rodents and similarly-sized mice
复制标题
DOI：
10.1093/gerona/61.10.1009
复制
发表时间：
2006-10-01
影响因子：
5.1
通讯作者：
Buffenstein, Rochelle
中科院分区：
文献类型：
--
作者：
Hulbert, A. J.;Faulks, Sally C.;Buffenstein, Rochelle
尽管膜磷脂对过氧化的脆弱性不同，但最大寿命（MLS）物种差异的根本原因尚不清楚。膜成分和寿命与体型相关；寿命较长、体型较大的哺乳动物的细胞膜含有较少的多不饱和脂肪酸 (PUFA)。我们通过气液色谱法测定了裸鼹鼠（MLS > 28.3 岁）和类似大小的小鼠（MLS = 3-4 岁）的膜磷脂组成，以评估是否可以解释类似于 9X MLS 的差异。鼹鼠的膜成分随着年龄的增长而变化。两个物种的膜总不饱和脂肪酸含量相似；然而，小鼠体内的二十二碳六烯酸 (DHA) 含量却高出 9 倍。由于这种 n-3PUFA 最容易受到脂质过氧化的影响，因此鼹鼠膜比小鼠膜更能抵抗氧化应激。根据肌肉和肝脏线粒体膜计算得出的裸鼹鼠过氧化指数与 MLS 而非体型预测的结果一致，表明膜磷脂组成是长寿的重要决定因素。
Underlying causes of species differences in maximum life span (MLS) are unknown, although differential vulnerability of membrane phospholipids to peroxidation is implicated. Membrane composition and longevity correlate with body size; membranes of longer-living, larger mammals have less polyunsaturated fatty acid (PUFA). We determined membrane phospholipid composition of naked mole-rats (MLS > 28.3 years) and similar-sized mice (MLS = 3-4 years) by gas-liquid chromatography to assess if the similar to 9X MLS difference could be explained. Mole-rat membrane composition was unchanged with age. Both species had similar amounts of membrane total unsaturated fatty acids; however, mice had 9 times more docosahexaenoic acid (DHA). Because this n-3PUFA is most susceptible to lipid peroxidation, mole-rat membranes are substantially more resistant to oxidative stress than are mice membranes. Naked mole-rat peroxidation indices, calculated from muscle and liver mitochondrial membranes, concur with those predicted by MLS rather than by body size, suggesting that membrane phospholipid composition is an important determinant of longevity.