Oxide-dependent adhesion of the Jurkat line of T lymphocytes.
Oxide-dependent adhesion of the Jurkat line of T lymphocytes.
复制标题
T 淋巴细胞 Jurkat 系的氧化物依赖性粘附。
DOI：
10.1021/la8040192
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Sahai,Nita
中科院分区：
文献类型：
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作者：
Stevens,MJ;Donato,LeslieJ;Lower,StevenK;Sahai,Nita
采用原子力显微镜（AFM）测定了Jurkat细胞在pH 7.2水溶液中6种金属氧化物表面(石英（α-SiO2）晶面、无定形sio2玻璃、金红石（α-TiO2）、白云母云母(KAl2（AlSi3O10)（OH)2）和多晶刚玉（α-Al2O3））上的粘附力。我们首次定量地表明，T淋巴细胞的粘附力和粘附功与底物的零电荷点相关，表明在负电荷较小的表面上吸附效果更好。粘附事件也表现出锯齿状的力-距离分布，表明蛋白质键。与氧化物Hamaker常数没有发现显著的相关性，表明范德华力的贡献可以忽略不计，也与表面粗糙度无关。这些结果表明，当细胞表面受体未被激活时，Jurkat细胞粘附主要由与模块化糖蛋白或其他非t细胞表面特有的蛋白质展开相关的特定相互作用以及负电荷糖蛋白和可变电荷氧化物表面之间的静电力所主导。我们的研究结果对免疫系统细胞与人体中存在的金属氧化物的相互作用具有启示意义，无论是在生物医学应用中设计的，还是在肺部吸入的矿物粉尘颗粒等无意中。
The adhesion force of Jurkat cells was measured using atomic force microscopy (AFM) in aqueous solution at pH 7.2 on six metal oxide surfaces, namely, two quartz (α-SiO2) crystal faces, amorphous SiO2glass, rutile (α-TiO2), muscovite mica (KAl2(AlSi3O10)(OH)2), and polycrystalline corundum (α-Al2O3). We show quantitatively for the first time that the T lymphocyte adhesion force and adhesion work correlates with substrate point of zero charge, indicating greater adsorption on surfaces with smaller negative charge. Adhesion events also exhibited sawtooth-shaped force−distance profiles indicative of protein bonds. No significant correlations were found with oxide Hamaker constants, indicating negligible contributions from van der Waals forces, nor with surface roughness. These results suggest that, when cell−surface receptors are not activated, Jurkat cell adhesion is dominated by specific interactions related to the unfolding of modular glycoproteins or other proteins that are not unique to T-cell surfaces and by electrostatic forces between negatively charged glycoproteins and variably charged oxide surfaces. Our results have implications for the interactions of immune system cells with metal oxides present in the human body either by design as in biomedical applications or inadvertently such as inhaled mineral dust particles in the lung.