Deadenylation of cytoplasmic mRNA by the mammalian Ccr4-Not complex
Deadenylation of cytoplasmic mRNA by the mammalian Ccr4-Not complex
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DOI：
10.1042/bst20120074
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发表时间：
2012-08-01
影响因子：
3.9
通讯作者：
Winkler, G. Sebastiaan
中科院分区：
文献类型：
--
作者：
Doidge, Rachel;Mittal, Saloni;Winkler, G. Sebastiaan
Ccr 4-Not复合物是真核细胞中存在的主要去腺苷酶因子之一。这种多亚基蛋白质复合物由哺乳动物细胞中至少7个稳定缔合的亚基组成，包括两个酶促去腺苷酸酶亚基：一个DEDD（Asp-Glu-Asp-Asp）型去腺苷酸酶（hocT 7/人Caf 1/Caf 1a或hocT 8/人Pop 2/Caf 1b/Cali）和一个EEP（内切核酸酶-外切核酸酶-磷酸酶）型酶（hocT 6/人Ccr 4/Ccr 4a或hocT 6L/人Ccr 4样/Ccr 4 b）。在这里，人Ccr 4-Not复合物在胞质去腺苷化的mRNA的作用进行了讨论，包括其招聘的mRNA和BTG/Tob蛋白的作用的机制。
The Ccr4-Not complex is one of the major deadenylase factors present in eukaryotic cells. This multi-subunit protein complex is composed of at least seven stably associated subunits in mammalian cells including two enzymatic deadenylase subunits: one DEDD (Asp-Glu-Asp-Asp)-type deadenylase (either CNOT7/human Caf1/Caf1a or CNOT8/human Pop2/Caf1b/Calif) and one EEP (endonuclease-exonuclease-phosphatase)-type enzyme (either CNOT6/human Ccr4/Ccr4a or CNOT6L/human Ccr4-like/Ccr4b). Here, the role of the human Ccr4-Not complex in cytoplasmic deadenylation of mRNA is discussed, including the mechanism of its recruitment to mRNA and the role of the BTG/Tob proteins.