Circulating tumor cells in early stage lung adenocarcinoma: a case series report and literature review
Circulating tumor cells in early stage lung adenocarcinoma: a case series report and literature review
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早期肺腺癌的循环肿瘤细胞：病例系列报告和文献综述。
DOI：
10.18632/oncotarget.15506
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发表时间：
2017-04-04
期刊：
影响因子：
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通讯作者：
Tan, Qun-You
中科院分区：
文献类型：
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作者：
Jin, Xu-Rui;Zhu, Lu-Yao;Tan, Qun-You
目的：监测早期肺腺癌患者的循环肿瘤细胞(CTCs)。结果：根据上皮间充质转化(EMT)生物标志物，CTCs可分为上皮型(E-)、间叶型(M-)和上皮型(E&M-)。健康对照组未检测到CTCs。然而，在A组中，17例(17/18)发现了CTC。E-CTC的检出率(5/18)低于M-CTC(10/18)或E&M-CTC(14/18)。高含量的M-CTCs易出现在肿瘤内2 cm处。在A组和B组中，肿瘤进展的所有患者的CTCs计数均显著增加(7/7)。肿瘤进展期患者术后CTCs水平或变化幅度均高于无病生存期患者(P<；0.01)。此外，CanPatrol(TM)检测的CTCs可以通过CytoploRare或Pep@MNPs进行验证。材料和方法：我们包括4个队列患者和20名健康对照。在队列A中，麻醉前通过新建立的方法CanPatrol(TM)检测CTC，并在手术后进行纵向监测。在队列B中，CTC在手术前没有被评估，但在手术后被纵向检测。为了进行验证，我们在C组和D组分别用CytoploRare和EPCAM(+)-CTCs检测FOLR(+)-CTCs和EPCAM(+)-CTCs。结论：在早期肺腺癌中可以检测到CTCs，甚至在原位腺癌中也可以检测到CTCs，CTCs检测可以有效地监测肿瘤的进展。高度侵袭性和侵袭性CTC的生物标记物的区分值得进一步深入研究。
Purpose: The study aimed to monitor circulating tumor cells (CTCs) in early stage lung adenocarcinoma patients.Results: CTCs were characterized and classified to epithelial (E-) CTCs, mesenchymal (M-) CTCs and epithelial-mesenchymal (E&M-) CTCs, as per epithelialmesenchymal transition(EMT) biomarkers. CTCs could not be found in healthy controls. However, in cohort A, CTCs were found in 17 (17/18) cases. Detection rate of E-CTCs was lower (5/18) compared with M-CTC (10/18) or E&M-CTC (14/18). Highly abundant M-CTCs were prone to being in the tumors > 2 cm. In cohorts A and B, CTCs count increased significantly in all patients with tumor progression (7/7). Higher CTCs level or change range could be found postoperatively in the patients with tumor progression, as compared with patients with disease free survival (P < 0.01). Additionally, CTCs detected by CanPatrol (TM) could be validated by CytoploRare or Pep@MNPs.Materials and Methods: We included four cohorts of patients and 20 healthy controls. In cohort A, CTCs were detected by a newly established approach, i.e., CanPatrol (TM), prior to anesthesia and monitored after operation longitudinally. In cohort B, CTCs were not assessed prior to operation, but were longitudinally detected after operation. For validation, we detected FOLR(+)-CTCs by using CytoploRare and EPCAM(+)-CTCs by using Pep@MNPs prior to operation, in cohorts C and D, respectively.Conclusion: CTCs can be detected in early stage lung adenocarcinoma, even in adenocarcinoma in situ, and CTCs detection can effectively monitor tumor progression. The distinguishing of biomarkers of highly invasive and aggressive CTCs warrants further robust study.