Microfabricated 384-lane capillary array electrophoresis bioanalyzer for ultrahigh-throughput genetic analysis
Microfabricated 384-lane capillary array electrophoresis bioanalyzer for ultrahigh-throughput genetic analysis
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DOI：
10.1021/ac020236g
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发表时间：
2002-10-01
影响因子：
7.4
通讯作者：
Mathies, RA
中科院分区：
文献类型：
--
作者：
Emrich, CA;Tian, HJ;Mathies, RA
研制了一种微加工的384泳道毛细管阵列电泳装置，并用于大规模并行遗传分析。384毛细管通道，排列在一个200毫米直径的玻璃基板三明治的中心径向，使用可扩展的微制造技术来自半导体行业。将样品装载到晶片周边的储存器中，在8厘米长的聚（二甲基丙烯酰胺）凝胶填充通道上分离，并用四色旋转共聚焦荧光扫描仪进行检测。该生物分析仪的性能和吞吐量通过在仅325 s内同时对384个个体进行人类HFE基因中常见的血色素沉着症相关H63D突变的基因分型来证明。这种芯片实验室设备充分利用了微加工的力量来生产高密度毛细管电泳阵列，并将其用于高通量生物分析。
A microfabricated 384-lane capillary array electrophoresis device is developed and utilized for massively parallel genetic analysis. The 384 capillary lanes, arrayed radially about the center of a 200-mm-diameter glass substrate sandwich, are constructed using scalable microfabrication techniques derived from the semiconductor industry. Samples are loaded into reservoirs on the perimeter of the wafer, separated on the 8-cm-long poly(dimethylacrylamide) gel-filled channels, and detected with a four-color rotary confocal fluorescence scanner. The performance and throughput of this bioanalyzer are demonstrated by simultaneous genotyping 384 individuals for the common hemochromatosis-linked H63D mutation in the human HFE gene in only 325 s. This lab-on-a-chip device thoroughly exploits the power of microfabrication to produce high-density capillary electrophoresis arrays and to use them for high-throughput bioanalysis.