Expression of nestin, desmin and vimentin in intact and regenerating muscle spindles of rat hind limb skeletal muscles
Expression of nestin, desmin and vimentin in intact and regenerating muscle spindles of rat hind limb skeletal muscles
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DOI：
10.1007/s00418-008-0523-7
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发表时间：
2009-02-01
影响因子：
2.3
通讯作者：
Mokry, Jaroslav
中科院分区：
文献类型：
--
作者：
Cizkova, Dana;Soukup, Tomas;Mokry, Jaroslav
我们描述了巢蛋白、去蛋白和波形蛋白在大鼠后肢骨骼肌完整肌梭和再生肌梭中的表达和分布规律。将15日龄大鼠指长伸肌（EDL）或比目鱼肌等肌内移植到成年雌性近交系Lewis大鼠指长伸肌中诱导再生。在存活7、16、21、29天后切除移植物宿主肌肉，进行免疫组织化学染色。巢蛋白在宿主肌肉完整纺锤体中的表达仅限于感觉和运动神经的雪旺细胞。然而，在移植肌肉中，在移植后7天和16天再生的“纺锤形纤维”中也发现了巢蛋白的表达。从第21天开始，再生的纺锤体纤维不再具有巢蛋白免疫反应性。在再生和完整纺锤体的所有发育阶段均检测到Desmin。Vimentin在所有肌纺锤体外、内囊细胞以及新生肌纺锤体新形成的成肌细胞和肌管中均有表达。我们的研究结果表明，这些中间纤维在再生纺锤体纤维中的表达模式与再生外渗纤维中的表达模式相一致，这支持了我们之前提出的它们类似于小直径外渗纤维的观点。
We describe the expression and distribution patterns of nestin, desmin and vimentin in intact and regenerating muscle spindles of the rat hind limb skeletal muscles. Regeneration was induced by intramuscular isotransplantation of extensor digitorum longus (EDL) or soleus muscles from 15-day-old rats into the EDL muscle of adult female inbred Lewis rats. The host muscles with grafts were excised after 7-, 16-, 21- and 29-day survival and immunohistochemically stained. Nestin expression in intact spindles in host muscles was restricted to Schwann cells of sensory and motor nerves. In transplanted muscles, however, nestin expression was also found in regenerating "spindle fibers", 7 and 16 days after grafting. From the 21st day onwards, the regenerated spindle fibers were devoid of nestin immunoreactivity. Desmin was detected in spindle fibers at all developmental stages in regenerating as well as in intact spindles. Vimentin was expressed in cells of the outer and inner capsules of all muscle spindles and in newly formed myoblasts and myotubes of regenerating spindles 7 days after grafting. Our results show that the expression pattern of these intermediate filaments in regenerating spindle fibers corresponds to that found in regenerating extrafusal fibers, which supports our earlier suggestion that they resemble small-diameter extrafusal fibers.