Application of Marker Assisted Selection for Potato Virus Y Resistance in the University of Wisconsin Potato Breeding Program
Application of Marker Assisted Selection for Potato Virus Y Resistance in the University of Wisconsin Potato Breeding Program
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DOI：
10.1007/s12230-015-9431-2
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发表时间：
2015-06-01
影响因子：
1.5
通讯作者：
Charkowski, Amy O.
中科院分区：
文献类型：
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作者：
Fulladolsa, Ana C.;Navarro, Felix M.;Charkowski, Amy O.
马铃薯Y病毒（PVY）是导致北美大部分种薯批次被拒绝的原因。一些商业栽培品种和育种系具有PVY抗性的主效基因，但它们在北美育种计划中的使用受到限制。PVY抗性分子标记辅助选择可以提高抗病植株的鉴定效率和抗病性整合到新品种中的效率。利用与Ry（adg）和Ry（sto）基因连锁的分子标记RYSC 3和YES 3 -3B，对46个育种无性系和品种进行了筛选，鉴定出19个抗病无性系。抗病亲本与农艺性状和市场性状均较好的感病亲本杂交，各家系的分子标记分析显示分离比为1：1。持续2年以上的选择和缺乏主要连锁阻力的育种家评级评估的分离比表明，没有证据表明PVY抗性与不良性状的关联。
Potato virus Y (PVY) is responsible for the majority of seed potato lot rejections in North America. Some commercial cultivars and breeding lines have major genes for PVY resistance, but their use in North American breeding programs has been limited. Marker assisted selection of PVY resistance can increase the efficiency of identifying resistant plants and integrating resistance into new cultivars. We used markers RYSC3 and YES3-3B, linked to genes Ry (adg) and Ry (sto) , respectively, to screen 46 breeding clones and cultivars, and identified 19 resistant clones. Resistant parents were crossed with susceptible parents with good market and agronomic traits and molecular marker analysis of each family showed a 1:1 segregation ratio. The persistence of this segregation ratio over 2 years of selection and the lack of major linkage drag as assessed by a breeder rating indicated that there was no evidence of an association of PVY resistance with undesirable traits.