Synergic Entrainment of Drosophila's Circadian Clock by Light and Temperature
Synergic Entrainment of Drosophila's Circadian Clock by Light and Temperature
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DOI：
10.1177/0748730409348551
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发表时间：
2009-12-01
影响因子：
3.5
通讯作者：
Helfrich-Foerster, Charlotte
中科院分区：
文献类型：
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作者：
Yoshii, Taishi;Vanin, Stefano;Helfrich-Foerster, Charlotte
在许多生物体中，每日的光和温度循环被认为是生物钟最重要的时间表。每一个单一的授时因子对生物钟的影响已经得到了很好的研究，但很少有人知道两个授时因子对生物钟的任何协同效应。在自然界中，光和温度表现出特征性的日振荡，温度在光照阶段上升，并在下午晚些时候达到最大值。在这里，我们研究了行为和分子的节奏在果蝇模拟自然低光暗（LD）和温度（T）的周期，通常发生在九月春分。野生型苍蝇进行模拟LD或T周期单独或两者的组合。在3种不同的条件下，在不同的时钟神经元的行为节律和分子节律进行了评估。虽然所有条件下都伴随有行为节律，但在LD和T周期的组合下，节律最稳健。钟神经元对LD和T周期的反应不同。一些不夹带由T周期单独;其他仅轻微夹带由LD周期单独。单独LD和单独T周期之间的分子循环的幅度没有不同;但是单独LD可以将起搏神经元设置到相似的相位，而单独T周期则不能。两个时代特征的结合导致了一切。时钟神经元不仅具有相似的相位，而且在大多数细胞中增强了Timeless循环的幅度。我们的研究结果表明，这两种授时因子协同地参与果蝇的行为和分子节律。
Daily light and temperature cycles are considered the most important zeitgebers for circadian clocks in many organisms. The influence of each single zeitgeber on the clock has been well studied, but little is known about any synergistic effects of both zeitgebers on the clock. In nature, light and temperature show characteristic daily oscillations with the temperature rising during the light phase and reaching its maximum in the late afternoon. Here, we studied behavioral and molecular rhythms in Drosophila melanogaster under simulated natural low light-dark (LD) and temperature (T) cycles that typically occur during the September equinox. Wild-type flies were either subjected to simulated LD or T cycles alone or to a combination of both. Behavioral rhythms and molecular rhythms in the different clock neurons were assessed under the 3 different conditions. Although behavioral rhythms entrained to all conditions, the rhythms were most robust under the combination of LD and T cycles. The clock neurons responded differently to LD and T cycles. Some were not entrained by T cycles alone; others were only slightly entrained by LD cycles alone. The amplitude of the molecular cycling was not different between LD alone and T cycles alone; but LD alone could set the pacemaker neurons to similar phases, whereas T cycles alone could not. The combination of the 2 zeitgebers entrained all. clock neurons not only with similar phase but also enhanced the amplitude of Timeless cycling in the majority of cells. Our results show that the 2 zeitgebers synergistically entrain behavioral and molecular rhythms of Drosophila melanogaster.