Molecular rheotaxis directs DNA migration and concentration against a pressure-driven flow.
Molecular rheotaxis directs DNA migration and concentration against a pressure-driven flow.
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DOI：
10.1038/s41467-017-01214-y
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发表时间：
2017-10-31
影响因子：
16.6
通讯作者：
Wang TH
中科院分区：
文献类型：
--
作者：
Friedrich SM;Burke JM;Liu KJ;Ivory CF;Wang TH
在线预富集技术用于提高微流控DNA分析平台的灵敏度。最常见的方法是电动的，需要外加电场。在这里，我们描述了一种微流控DNA预浓缩技术，不需要一个外部的领域。相反，从流体填充的微毛细管到较低离子强度DNA样品储器的压力驱动流动诱导逆流动方向的自发DNA迁移。我们称之为分子流变性的这种迁移现象在几秒钟内启动，并导致毛细血管口处的浓缩DNA团块。我们证明了这种浓缩方法可以很容易地集成到一个微流控总分析系统组成的在线DNA预浓缩，大小分离，和单分子检测。配对的实验和数值模拟结果被用来描绘诱导分子流变性所需的参数，阐明潜在的机制，并优化条件，以实现DNA浓度因子超过10,000倍。实施核酸预浓缩方法可以提高微流体分析系统的灵敏度。在这里，Friedrich等人使用压力驱动流将DNA浓缩了许多数量级，这可能导致一个简单实用的微量分析平台。
In-line preconcentration techniques are used to improve the sensitivity of microfluidic DNA analysis platforms. The most common methods are electrokinetic and require an externally applied electric field. Here we describe a microfluidic DNA preconcentration technique that does not require an external field. Instead, pressure-driven flow from a fluid-filled microcapillary into a lower ionic strength DNA sample reservoir induces spontaneous DNA migration against the direction of flow. This migratory phenomenon that we call Molecular Rheotaxis initiates in seconds and results in a concentrated DNA bolus at the capillary orifice. We demonstrate the ease with which this concentration method can be integrated into a microfluidic total analysis system composed of in-line DNA preconcentration, size separation, and single-molecule detection. Paired experimental and numerical simulation results are used to delineate the parameters required to induce Molecular Rheotaxis, elucidate the underlying mechanism, and optimize conditions to achieve DNA concentration factors exceeding 10,000 fold. Implementing a nucleic acid preconcentration method can improve the sensitivity of microfluidic analysis systems. Here Friedrich et al. concentrate DNA by many orders of magnitude using pressure-driven flow, which could lead to a simple and practical microanalysis platform.
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