Ogura-CMS in Chinese cabbage (Brassica rapa ssp pekinensis) causes delayed expression of many nuclear genes
Ogura-CMS in Chinese cabbage (Brassica rapa ssp pekinensis) causes delayed expression of many nuclear genes
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DOI：
10.1016/j.plantsci.2012.11.001
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发表时间：
2013-02-01
期刊：
影响因子：
5.2
通讯作者：
Hur, Yoonkang
中科院分区：
文献类型：
--
作者：
Dong, Xiangshu;Kim, Wan Kyu;Hur, Yoonkang
研究了甘蓝型油菜细胞质雄性不育的调控机制。利用B. rapa 300-K寡聚探针（Br 300 K）微阵列。Ogura-CMS大白菜产生很少和不育的花粉粒不开裂花药。与保持系相比，CMS植株花丝短，植株生长短，开花和花粉发育延迟。微阵列分析表明，4646个基因在Ogura-CMS和保持系之间表现出不同的表达，这取决于花芽大小。在Ogura-CMS及其保持系中分别发现了108个和62个特异表达基因。Ogura-CMS的胰岛特异性基因包括胁迫相关基因、氧化还原相关基因和B基因。rapa新基因在保持系中，与花粉衣和萌发相关的基因在长于3 mm的花芽中特异性表达，表明这些基因在Ogura-CMS中的表达不足与花粉功能障碍直接相关。此外，许多与生长素反应、ATP合成、花粉发育和胁迫反应相关的核基因在Ogura-CMS植物中的表达比保持系延迟，这与Ogura-CMS植物生长发育延迟一致。延迟表达可能减少花粉粒产生和/或导致不育，这意味着线粒体，逆行信号延迟核基因表达。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
We investigated the mechanism regulating cytoplasmic male sterility (CMS) in Brassica rapa ssp. pekinensis using floral bud transcriptome analyses of Ogura-CMS Chinese cabbage and its maintainer line in B. rapa 300-K oligomeric probe (Br300K) microarrays. Ogura-CMS Chinese cabbage produced few and infertile pollen grains on indehiscent anthers. Compared to the maintainer line, CMS plants had shorter filaments and plant growth, and delayed flowering and pollen development. In microarray analysis, 4646 genes showed different expression, depending on floral bud size, between Ogura-CMS and its maintainer line. We found 108 and 62 genes specifically expressed in Ogura-CMS and its maintainer line, respectively. Ogura-CMS lisle-specific genes included stress-related, redox-related, and B. rapa novel genes. In the maintainer line, genes related to pollen coat and germination were specifically expressed in floral buds longer than 3 mm, suggesting insufficient expression of these genes in Ogura-CMS is directly related to dysfunctional pollen. In addition, many nuclear genes associated with auxin response, ATP synthesis, pollen development and stress response had delayed expression in Ogura-CMS plants compared to the maintainer line, which is consistent with the delay in growth and development of Ogura-CMS plants. Delayed expression may reduce pollen grain production and/or cause sterility, implying that mitochondrial, retrograde signaling delays nuclear gene expression. (C) 2012 Elsevier Ireland Ltd. All rights reserved.