Erv41p and Erv46p: new components of COPII vesicles involved in transport between the ER and Golgi complex.
Erv41p and Erv46p: new components of COPII vesicles involved in transport between the ER and Golgi complex.
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ERV41P和ERV46P：涉及ER和高尔基体复合物之间运输的Copii囊泡的新成分。
DOI：
10.1083/jcb.152.3.503
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发表时间：
2001-02-05
期刊：
影响因子：
--
通讯作者：
Barlowe C
中科院分区：
文献类型：
--
作者：
Otte S;Belden WJ;Heidtman M;Liu J;Jensen ON;Barlowe C
用基质辅助激光解吸电离质谱仪结合数据库检索对纯化的COPII囊泡上的蛋白质进行分析。我们鉴定了4个已知的囊泡蛋白(Erv14p、Bet1p、Emp24p和Erv25p)和另外9个未定位于内质网小泡的物种(Yip3p、Rer1p、Erp1p、Erp2p、Erv29p、Yif1p、Erv41p、Erv46p和Emp47p)。使用抗体，我们证明了这些蛋白质被选择性地和有效地包装到COPII囊泡中。其中三个新发现的囊泡蛋白(Erv29p、Erv41p和Erv46p)代表未知的完整膜蛋白，它们在物种中是保守的。对Erv41p和Erv46p进行了进一步鉴定。这些蛋白共定位于内质网和高尔基体膜，并存在于通过免疫沉淀分离的洗涤剂可溶复合体中。缺少Erv41p和/或Erv46p的酵母菌株是活的，但表现出冷敏感性。Erv41p和Erv46p的表达水平是相互依赖的，以至于Erv41Δ株中Erv46p表达降低，而Erv46Δ株中未检测到Erv41p。当erv41Δ或ev46Δ等位基因与早期分泌途径中的其他突变相结合时，在一些双突变株中观察到生长表型的改变。复制内质网和高尔基体之间运输的无细胞分析表明，Erv41p-Erv46p复合体的缺失影响膜融合运输阶段。
Proteins contained on purified COPII vesicles were analyzed by matrix-assisted laser desorption ionization mass spectrometry combined with database searching. We identified four known vesicle proteins (Erv14p, Bet1p, Emp24p, and Erv25p) and an additional nine species (Yip3p, Rer1p, Erp1p, Erp2p, Erv29p, Yif1p, Erv41p, Erv46p, and Emp47p) that had not been localized to ER vesicles. Using antibodies, we demonstrate that these proteins are selectively and efficiently packaged into COPII vesicles. Three of the newly identified vesicle proteins (Erv29p, Erv41p, and Erv46p) represent uncharacterized integral membrane proteins that are conserved across species. Erv41p and Erv46p were further characterized. These proteins colocalized to ER and Golgi membranes and exist in a detergent-soluble complex that was isolated by immunoprecipitation. Yeast strains lacking Erv41p and/or Erv46p are viable but display cold sensitivity. The expression levels of Erv41p and Erv46p are interdependent such that Erv46p was reduced in an erv41Δ strain, and Erv41p was not detected in an erv46Δ strain. When the erv41Δ or ev46Δ alleles were combined with other mutations in the early secretory pathway, altered growth phenotypes were observed in some of the double mutant strains. A cell-free assay that reproduces transport between the ER and Golgi indicates that deletion of the Erv41p–Erv46p complex influences the membrane fusion stage of transport.
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影响因子：
4.8
作者：
Belden, WJ;Barlowe, C
通讯作者：
Barlowe, C
影响因子：
3.5
作者：
Cho, JH;Noda, Y;Yoda, K
通讯作者：
Yoda, K
DOI：
10.1083/jcb.149.1.55
发表时间：
2000-04-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cao X;Barlowe C
通讯作者：
Barlowe C
影响因子：
11.4
作者：
JACKSON, MR;NILSSON, T;PETERSON, PA
通讯作者：
PETERSON, PA
影响因子：
64.5
作者：
Bednarek, SY;Ravazzola, M;Orci, L
通讯作者：
Orci, L