A differential scanning calorimetry study of acetylcholine receptor-rich membranes from Torpedo californica.
A differential scanning calorimetry study of acetylcholine receptor-rich membranes from Torpedo californica.
复制标题
加州鱼雷富含乙酰胆碱受体的膜的差示扫描量热法研究。
DOI：
10.1016/s0021-9258(18)32600-0
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
M. Martinez
中科院分区：
文献类型：
--
作者：
M. Farach;M. Martinez
使用差示扫描量热法结合热变性材料的凝胶电泳分析和经过离散转变后的功能测定技术，对来自加州鱼雷电板组织的各种富含乙酰胆碱受体的膜制剂进行了检查。在未分级的膜中，观察到四个不可逆的量热转变，其中之一（Td=59℃）可以归因于乙酰胆碱受体功能的完全丧失。第二个较低温度转变显然对应于某些外周膜蛋白的损失，包括 Mr = 43,000 多肽和乙酰胆碱酯酶活性。高度富集乙酰胆碱受体多肽的膜制剂在 59 摄氏度下包含一个主要转变，这可能表明该转变对乙酰胆碱受体的添加配体的存在敏感，支持其与受体蛋白的结构改变或其在膜中的排列有关。
Various acetylcholine receptor-rich membrane preparations from Torpedo californica electroplax tissue were examined using the techniques of differential scanning calorimetry coupled with gel electrophoretic analysis of heat-denaturing material and functional assays following passage through discrete transitions. In unfractionated membranes, four irreversible calorimetric transitions were observed, one of which (Td = 59 degrees C) could be assigned to a complete loss of acetylcholine receptor function. A second lower temperature transition apparently corresponds to loss of certain peripheral membrane proteins including the Mr = 43,000 polypeptide and the acetylcholinesterase activity. Membrane preparations highly enriched in acetylcholine receptor polypeptides contained a major transition at 59 degrees C which could be shown to be sensitive to the presence of added ligands of the acetylcholine receptor, supporting its assignment to structural alterations of the receptor protein or its arrangement in the membrane.