The electrochemical monitoring of the perturbation of charge transfer through DNA by cisplatin.
The electrochemical monitoring of the perturbation of charge transfer through DNA by cisplatin.
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DOI：
10.1021/ja0723075
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发表时间：
2007-06
影响因子：
15
通讯作者：
E. Wong;J. Gooding
中科院分区：
文献类型：
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作者：
E. Wong;J. Gooding
我们提出了一种新的电化学策略来探测抗癌药物顺铂与 DNA 在液固界面的结合。该策略利用通过双链 DNA (dsDNA) 观察到的长程电荷转移的敏感性，利用药物结合时电荷转移的“开”和“关”切换作为探测顺铂暴露后 DNA 发生的电子变化的基础。当氧化还原活性物质插入 dsDNA 碱基对 π 堆栈时，就会发生电荷转移，从而产生电流。 DNA 碱基对堆栈的破坏将降低电荷转移到氧化还原物质的效率，因此观察到长程电荷转移产生的电流减少。因此，电化学技术可以提供有关 DNA 双链体固有电子特性破坏的信息，这为研究药物与 DNA 结合提供了一种新方法。
We present a new electrochemical strategy to probe the binding of the anticancer drug, cisplatin, with DNA at the liquid−solid interface. The strategy exploits the sensitivity of a long-range charge transfer observed through double stranded DNA (dsDNA), utilizing “on” and “off” switching of such a charge transfer upon drug binding as the basis to probe the electronic changes that occur to the DNA upon cisplatin exposure. The charge transfer occurs when a redox active species intercalates into the dsDNA base-pair π-stack, thereby producing a current. Disruption of the DNA base-pair stack will reduce the efficiency of the charge transfer to the redox species, therefore a diminution in the current arising from the long-range charge transfer is observed. As a result, the electrochemical technique can provide information on the disruption of the intrinsic electronic properties of the DNA duplexes, which provide a new way to study drug−DNA binding.