Cytokine profile in the liver of primary biliary cirrhosis
Cytokine profile in the liver of primary biliary cirrhosis
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DOI：
10.1023/a:1020511002025
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发表时间：
1999-11-01
影响因子：
9.1
通讯作者：
Tsuji, T
中科院分区：
文献类型：
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作者：
Nagano, T;Yamamoto, K;Tsuji, T
我们对原发性胆汁性肝硬化 (PBC) 患者肝脏中的细胞因子谱进行了表征。从 9 名早期 PBC 患者、10 名慢性丙型肝炎 (CHC) 患者和 4 名正常对照患者的活检标本中提取总 RNA。使用干扰素 (IFN)-γ 和白细胞介素 (IL)-2、-4、-5、-6、-10、-12 (p40) 和 -15 的引物，通过聚合酶链式反应制备和扩增 cDNA。大多数 PBC 病例表达细胞因子，如 IFN-γ 和 IL-5、-6、-10、-12 和 -15。 IL-5 和 IL-6 的表达率高于 CHC 和对照。 PBC中IL-5的较高表达率与嗜酸性粒细胞浸润相关。 IL-2和IL-4很少被检测到。半定量分析显示，PBC和CHC中IFN-γ和IL-10的表达发生逆转：PBC中IFN-γ高、IL-10低，CHC中IL-10高、IFN-γ低。这些结果表明细胞因子表达在 PBC 中存在偏差，Th1 和 Th2 细胞因子可能在发病机制中发挥作用。
We characterized the cytokine profile in the liver of patients with primary biliary cirrhosis (PBC). Total RNA was extracted from the biopsy specimens of 9 patients with early-stage PBC, 10 with chronic hepatitis C (CHC), and 4 normal controls. cDNA was prepared and amplified with a polymerase chain reaction using primers for interferon (IFN)-gamma and interleukin (IL)-2, -4, -5, -6, -10, -12 (p40), and -15. Cytokines such as IFN-gamma and IL-5, -6, -10, -12, and -15 were expressed in most cases of PBC. Expression rates of IL-5 and IL-6 were higher than in CHC and controls. The higher expression rate of IL-5 in PBC was associated with eosinophil infiltration. IL-2 and IL-4 were rarely detected. Semiquantitative analysis revealed that the expression of IFN-gamma and IL-10 was reversed in PBC and CHC: high IFN-gamma and low IL-10 in PBC and high IL-10 and low IFN-gamma in CHC. These results suggest that cytokine expression is skewed in PBC and both Th1 and Th2 cytokines may play a role in the pathogenesis.