Branched RNA nanostructures for RNA interference
Branched RNA nanostructures for RNA interference
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DOI：
10.1039/c1cc11780g
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发表时间：
2011-01-01
影响因子：
4.9
通讯作者：
Ito, Yoshihiro
中科院分区：
文献类型：
--
作者：
Nakashima, Yuko;Abe, Hiroshi;Ito, Yoshihiro
通过组装单链RNA进行RNA干扰，设计了具有三路或四路连接的分枝RNA。人类将分支RNA转化为约20个碱基对的双链RNA，这是一种标准的siRNA物种。我们的四聚体设计在5天内提供了强大的沉默效果。
Branched RNAs with three- or four-way junctions were designed by assembling single-stranded RNA for RNA interference. Human Dicer transformed branched RNAs into about 20 base pairs of double-stranded RNA, which is a standard siRNA species. Our tetramer design provides a potent silencing effect over a period of 5 days.