Bacterial Community Structure and Diversity in a Century-Old Manure-Treated Agroecosystem
Bacterial Community Structure and Diversity in a Century-Old Manure-Treated Agroecosystem
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DOI：
10.1128/aem.70.10.5868-5874.2004
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发表时间：
2004-10
影响因子：
4.4
通讯作者：
H. Sun;S. Deng;W. Raun
中科院分区：
文献类型：
--
作者：
H. Sun;S. Deng;W. Raun
土壤微生物群落结构和多样性的变化可以反映环境影响。采用PCR扩增和变性梯度凝胶电泳（PCR-DGGE）分离技术，研究了6个农业生态系统中细菌群落的16 SrRNA基因指纹。这些土壤用粪肥处理了世纪，或用不同的肥料处理了70多年。PCR-DGGE带型的变化表明，土壤管理措施对土壤细菌群落结构和多样性有影响。施用有机肥的土壤中细菌群落结构与未施用有机肥的土壤中细菌群落结构的关系比施用无机肥的土壤中的关系更为密切。石灰处理对细菌群落结构影响不大。与其他处理相比，P和N-P处理的土壤细菌群落结构之间的关系更为密切。在测试的土壤中，一个显着较高的细菌核糖体的数量和更均匀的细菌群落分布存在于粪肥处理的土壤。从经过世纪粪肥处理的土壤中获得的99个克隆中，有2个（均为假单胞菌属）与GenBank数据库中的序列相似性为100%。其中两个克隆可能是嵌合体。根据相似性匹配，其余97个克隆形成6个主要的集群。五十六的97个被指定为分类单位，分为五个主要类群：α-，β-和γ-变形菌（36个克隆），酸杆菌（16个克隆），拟杆菌（2个克隆），硝化螺菌（1个克隆），和厚壁菌门（1个克隆）。41个克隆体仍然未分类。从这项研究的结果表明，细菌群落结构是密切相关的农业生态系统管理措施进行了70多年。
ABSTRACT Changes in soil microbial community structure and diversity may reflect environmental impact. We examined 16S rRNA gene fingerprints of bacterial communities in six agroecosystems by PCR amplification and denaturing gradient gel electrophoresis (PCR-DGGE) separation. These soils were treated with manure for over a century or different fertilizers for over 70 years. Bacterial community structure and diversity were affected by soil management practices, as evidenced by changes in the PCR-DGGE banding patterns. Bacterial community structure in the manure-treated soil was more closely related to the structure in the untreated soil than that in soils treated with inorganic fertilizers. Lime treatment had little effect on bacterial community structure. Soils treated with P and N-P had bacterial community structures more closely related to each other than to those of soils given other treatments. Among the soils tested, a significantly higher number of bacterial ribotypes and a more even distribution of the bacterial community existed in the manure-treated soil. Of the 99 clones obtained from the soil treated with manure for over a century, two (both Pseudomonas spp.) exhibited 100% similarity to sequences in the GenBank database. Two of the clones were possible chimeras. Based on similarity matching, the remaining 97 clones formed six major clusters. Fifty-six out of 97 were assigned taxonomic units which grouped into five major taxa: α-, β-, and γ-Proteobacteria (36 clones), Acidobacteria (16 clones), Bacteroidetes (2 clones), Nitrospirae (1 clone), and Firmicutes (1 clone). Forty-one clones remained unclassified. Results from this study suggested that bacterial community structure was closely related to agroecosystem management practices conducted for over 70 years.