STUDIES ON INDUCED CELLULAR AUTOPHAGY .2. CHARACTERIZATION OF MEMBRANES BORDERING AUTOPHAGOSOMES IN PARENCHYMAL LIVER CELLS
STUDIES ON INDUCED CELLULAR AUTOPHAGY .2. CHARACTERIZATION OF MEMBRANES BORDERING AUTOPHAGOSOMES IN PARENCHYMAL LIVER CELLS
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DOI：
10.1016/0014-4827(69)90030-5
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发表时间：
1969-01-01
影响因子：
3.7
通讯作者：
ERICSSON, JL
中科院分区：
文献类型：
--
作者：
ERICSSON, JL
肾近曲小管细胞和肝细胞中的溶酶体用电子致密标记物进行标记。在经过自噬诱导处理（肾小管细胞腹腔注射杆菌肽；肝细胞部分肝切除和腹腔注射胰高血糖素）的细胞中研究了这种预先存在的标记溶酶体与形成和早期细胞隔离体（自噬空泡、细胞溶酶体）之间的关系。新形成的含有未改变细胞器的细胞隔离体在空间上与预先存在的标记溶酶体，似乎代表缺乏溶酶体酶的自噬体类型的“前溶酶体”。后来，明显的自噬体似乎与预先存在的标记溶酶体融合。这些发现与自噬体-溶酶体融合相一致，即溶酶体酶与隔离的细胞质细胞器接触以进行后续消化的机制。
Lysosomes in renal proximal tubule cells and hepatocytes were labelled with electron dense markers. The relationship between such preexisting labelled lysosomes, and forming and early cytosegresomes (autophagic vacuoles, cytolysosomes), was studied in cells subjected to autophagy-inducing treatments (i.p. injected bacitracin for renal tubule cells; partial hepatectomy and i.p. injected glucagon for hepatocytes).Newly formed cytosegresomes containing unaltered organelles were spatially unrelated to preexisting labelled lysosomes and appeared to represent “pre-lysosomes” of the autophagosome type lacking lysosomal enzyme. Later, the apparent autophagosomes seemed to fuse with preexisting labelled lysosomes.The findings are compatible with autophagosome-lysosome fusion as the mechanism whereby lysosomal enzymes are brought in contact with sequestered cytoplasmic organelles for subsequent digestion.