LEUKOTRIENE-B4 ACTION ON ENDOTHELIUM MEDIATES AUGMENTED NEUTROPHIL ENDOTHELIAL ADHESION
LEUKOTRIENE-B4 ACTION ON ENDOTHELIUM MEDIATES AUGMENTED NEUTROPHIL ENDOTHELIAL ADHESION
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DOI：
10.1073/pnas.81.7.2191
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
LEWIS, RA
中科院分区：
文献类型：
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作者：
HOOVER, RL;KARNOVSKY, MJ;LEWIS, RA
用白三烯B4 （LTB4）预处理牛内皮细胞，可增加人多形核白细胞（PMN）对单层内皮细胞的附着。反应是剂量依赖性的，在10-6 M时达到最大值，需要不超过1分钟的预孵化时间才能达到最大效果，一旦LTB4被去除，在30分钟内线性衰减。PMN与LTB4的预孵育不会增加对塑料基质或未经处理的内皮细胞单层的粘附，并消除对LTB4预处理的内皮细胞的增强粘附。在粘附相互作用期间同时暴露于两种细胞类型会产生双相剂量相关的增量，这表明对中性粒细胞粘附的抑制作用不如预处理间隔明显。因此，LTB4依赖性PMN对内皮细胞的粘附是由内皮细胞选择性介导的，是剂量相关的，在从内皮细胞中去除激动剂后是可逆的，并且LTB4对PMN的直接作用是相反的。
The attachment of human polymorphonuclear leukocytes (PMN) to bovine endothelial monolayers is increased by pretreatment of the endothelial cells with leukotriene B4 (LTB4). The response is dose dependent with a maximum at 10-6 M, requires no more than 1 min of preincubation for the maximal effect and decays linearly over 30 min once the LTB4 is removed. Preincubation of PMN with LTB4 does not augment subsequent adhesion to a plastic substratum or an untreated monolayer of endothelial cells and eliminates the augmented attachment to LTB4-pretreated endothelial cells. The simultaneous exposure of both cell types during the adhesion interaction gives a biphasic dose-related increment, suggesting that the inhibition of the neutrophil adherence is less marked than with a pretreatment interval. Thus, the LTB4-dependent adherence of PMN to endothelial cells is selectively mediated by endothelial cells, is dose related, is reversible upon removal of the agonist from the endothelial cells, and is opposed by the direct action of LTB4 on the PMN.