SUCCESSFUL 48‐HOUR PRESERVATION OF THE RAT LIVER BY CONTINUOUS HYPOTHERMIC PERFUSION WITH HAEMACCEL‐ISOTONIC CITRATE SOLUTION
SUCCESSFUL 48‐HOUR PRESERVATION OF THE RAT LIVER BY CONTINUOUS HYPOTHERMIC PERFUSION WITH HAEMACCEL‐ISOTONIC CITRATE SOLUTION
复制标题
使用 HAEMACCEL 等渗柠檬酸盐溶液连续低温灌注成功保存大鼠肝脏 48 小时
作者：
T. Tamaki;N. Kamada;D. Wight;D. Pegg
使用连续非脉动灌注方法，将11个大鼠肝脏在7°C下储存24或48小时。灌注液基于等渗柠檬酸盐保存溶液，但另外含有明胶多肽（Haemaccel，Hoechst）和氟碳乳剂（FC-43，绿色Cross Corp.）。同种异体肝脏保存后原位移植，长期存活率为3/6保存后24小时和4/5保存后48小时。血运重建后立即进行的所有活检组织学正常。在两个月时处死7只存活动物，组织学显示胆道梗阻，但在所有情况下，肝细胞似乎保存良好。这些晚期组织学发现在任何使用这种移植模型中都是常见的，并且被认为与难以获得满意的胆管吻合术有关。本文所述的灌注液能够提供可靠的大鼠肝脏48小时保存。
Eleven rat livers were stored at 7°C for 24 or 48 hr using a continuous nonpulsatile perfusion method. The perfusate was based on the isotonic citrate preservation solution but contained, in addition, gelatin polypeptides (Haemaccel, Hoechst) and fluorocarbon emulsion (FC-43, Green Cross Corp.). Isologous livers were orthotopically transplanted after preservation, and long-term survival was 3/6 following 24 hr preservation and 4/5 following 48 hr preservation. All the biopsies taken immediately after re-vascularization were histologically normal. The seven surviving animals were killed at two months and histology showed biliary obstruction, but in all cases the hepatocytes appeared to be well preserved. These late histological findings are common in any use of this transplantation model and are believed to be associated with the difficulty of obtaining a satisfactory anastomosis of the bile duct. The perfusate described here is capable of providing reliable 48 hr preservation of the rat liver.