An integrated immunoproteomics and bioinformatics approach for the analysis of Schistosoma japonicum tegument proteins
An integrated immunoproteomics and bioinformatics approach for the analysis of Schistosoma japonicum tegument proteins
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DOI：
10.1016/j.jprot.2014.01.010
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发表时间：
2014-02-26
影响因子：
3.3
通讯作者：
Zhou, Xiao-Nong
中科院分区：
文献类型：
--
作者：
Chen, Jun-Hu;Zhang, Ting;Zhou, Xiao-Nong
血吸虫病仍然是导致热带国家居民发病的主要被忽视的热带病之一。在研制疫苗方面已经作出了很大努力，因为人们认识到，疫苗可以作为今后控制和消除血吸虫病的化疗之外的重要补充成分。为了加速挖掘新的潜在靶抗原，必须使用蛋白质组学-微阵列技术以高通量的方式广泛和深入地搜索免疫原性蛋白。本研究采用免疫蛋白质组学和生物信息学相结合的方法对人血吸虫日本血吸虫的被膜进行了分析。结果表明，该全长被膜蛋白可高通量克隆、表达，并可在日本血吸虫感染患者和正常人血清中进行蛋白阵列筛选。首次鉴定出30种高免疫反应性被膜蛋白和10种AUC值大于0.90的抗原。其中免疫活性最高的被膜蛋白STIP1表现出良好的抗原性和免疫原性，成为设计抗寄生虫药物或疫苗的潜在靶点。血吸虫被膜在宿主与寄生虫的相互作用中起着至关重要的作用，有几种被膜蛋白被证明是潜在的候选疫苗。然而，目前还没有疫苗，因此从血吸虫被膜蛋白质组中鉴定新的靶抗原是很重要的。本文采用免疫蛋白质组学和生物信息学方法对日本血吸虫被膜蛋白进行了综合分析。首次鉴定出30种高免疫反应性被膜蛋白和10种ADC值大于0.90的抗原。特别地，我们发现17个被皮免疫蛋白质组与日本血吸虫的其他蛋白质具有推测的相互作用网络。该结果将为血吸虫病疫苗开发提供潜在靶点分子线索，并为血吸虫病诊断提供生物标志物。(C) 2014年作者。这是一篇基于CC by-nc-nd许可（http://creativecommons.org/licenses/by-nc-nd/3.0/）的开放获取文章。
Schistosomiasis remains one of the major neglected tropical diseases (NTDs) causing morbidity of humans residing in the tropical countries. Much effort has been devoted to the development of vaccines, since it is recognized that vaccines can be served as an important supplementary component alongside chemotherapy for the future control and elimination of schistosomiasis. To accelerate digging new potential target antigens, it is essential to extensively and intensively search immunogenic proteins in a high-throughput manner using proteomics-microarray techniques. In the present study, an integrated immunoproteomics and bioinformatics approach was used to profile the tegument of the human blood fluke Schistosoma japonicum. Results showed that the full-length tegument proteins were high-throughput cloned and expressed and screened with sera from S. japonicum-infected patients and normal subjects using protein arrays. Here, thirty highly immunoreactive tegument proteins and 10 antigens with an AUC value greater than 0.90 were identified at first time. In particularly, STIP1, the highest immunoreactive tegument protein has been shown good antigenicity and immunogenicity, and thus makes it to be a potential target for designing anti-parasite drug or vaccine.Biological significanceThe schistosome tegument plays a crucial role in host parasite interactions and there are several tegument proteins that proved to be potential vaccine candidates. However, vaccines are not yet available, thus it is important to identify new target antigens from schistosome tegument proteome. Herein, we demonstrate that the S. japonicum tegument proteins were analyzed by an integrated immunoproteomics and bioinformatics approach. We found that thirty highly immunoreactive tegument proteins and 10 antigens with an ADC value greater than 0.90 were identified for the first time. In particularly, we found 17 of tegument immunoproteomes having putative interaction networks with other proteins of S. japonicum. The results will provide clues of potential target molecules for vaccine development and biomarkers for diagnostics of schistosomiasis. (C) 2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).