Probiotic bacterium prevents cytokine-induced apoptosis in intestinal epithelial cells
Probiotic bacterium prevents cytokine-induced apoptosis in intestinal epithelial cells
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DOI：
10.1074/jbc.m207050200
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发表时间：
2002-12-27
影响因子：
4.8
通讯作者：
Polk, DB
中科院分区：
文献类型：
--
作者：
Yan, F;Polk, DB
益生菌是通过预防或改善疾病而有益于宿主的微生物。然而，很少有关于使用益生菌作为替代药物的科学依据的信息。本文旨在探讨益生菌对肠道细胞稳态的有益作用机制。我们现在报道一种这样的益生菌，鼠李糖乳杆菌GG (LGG)，在两种不同的肠上皮细胞模型中阻止细胞因子诱导的凋亡。用小鼠或人结肠细胞培养LGG可激活抗凋亡的Akt/蛋白激酶b。该模型益生菌还可通过肿瘤坏死因子、白细胞介素-1 α或γ -干扰素抑制促凋亡的p38/丝裂原活化的蛋白激酶的活化。此外，从LGG培养液上清中回收的产物显示Akt的浓度依赖性激活和细胞因子诱导的细胞凋亡的抑制。这些观察结果表明，在促凋亡细胞因子的环境中，益生菌微生物和上皮之间存在一种新的通讯机制，可以提高肠道细胞的存活率。
Probiotic bacteria are microorganisms that benefit the host by preventing or ameliorating disease. However, little information is known regarding the scientific rationale for using probiotics as alternative medicine. The purpose of this paper is to investigate the mechanisms of probiotic beneficial effects on intestinal cell homeostasis. We now report that one such probiotic, Lactobacillus rhamnosus GG (LGG), prevents cytokine-induced apoptosis in two different intestinal epithelial cell models. Culture of LGG with either mouse or human colon cells activates the anti-apoptotic Akt/protein kinase B. This model probiotic also inhibits activation of the pro-apoptotic p38/mitogen-activated protein kinase by tumor necrosis factor, interieukin-1alpha, or gamma-interferon. Furthermore, products recovered from LGG culture broth supernatant show concentration-dependent activation of Akt and inhibition of cytokine-induced apoptosis. These observations suggest a novel mechanism of communication between probiotic microorganisms and epithelia that increases survival of intestinal cells normally found in an environment of pro-apoptotic cytokines.