Sensing of Amino Acids in a Dopaminergic Circuitry Promotes Rejection of an Incomplete Diet in Drosophila
Sensing of Amino Acids in a Dopaminergic Circuitry Promotes Rejection of an Incomplete Diet in Drosophila
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DOI：
10.1016/j.cell.2013.12.024
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发表时间：
2014-01-30
期刊：
影响因子：
64.5
通讯作者：
Leopold, Pierre
中科院分区：
文献类型：
--
作者：
Bjordal, Marianne;Arquier, Nathalie;Leopold, Pierre
大脑是食物摄入的中央组织者，使食物来源的质量和数量与生物体的需求相匹配。为了确保适当的氨基酸平衡，许多物种拒绝缺乏一种或几种必需氨基酸（EAA）的饮食，并寻求更好的食物来源。在这里，我们表明，在果蝇幼虫中，这种行为依赖于大脑多巴胺能（DA）神经元中氨基酸的先天感知。我们证明，氨基酸传感器GCN 2的行为上游的GABA信号在DA神经元，以促进避免EAA缺乏饮食。使用实时钙成像在幼虫的大脑中，我们表明，氨基酸不平衡诱导快速和可逆的激活三个DA神经元，是必要的和足够的食物排斥。总之，这些数据确定了在特定DA神经元中操作并控制食物摄入的中央氨基酸传感机制。
The brain is the central organizer of food intake, matching the quality and quantity of the food sources with organismal needs. To ensure appropriate amino acid balance, many species reject a diet lacking one or several essential amino acids (EAAs) and seek out a better food source. Here, we show that, in Drosophila larvae, this behavior relies on innate sensing of amino acids in dopaminergic (DA) neurons of the brain. We demonstrate that the amino acid sensor GCN2 acts upstream of GABA signaling in DA neurons to promote avoidance of the EAA-deficient diet. Using real-time calcium imaging in larval brains, we show that amino acid imbalance induces a rapid and reversible activation of three DA neurons that are necessary and sufficient for food rejection. Taken together, these data identify a central aminoacid- sensing mechanism operating in specific DA neurons and controlling food intake.