Spontaneous and cisplatin-induced recombination in Escherichia coli
Spontaneous and cisplatin-induced recombination in Escherichia coli
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DOI：
10.1016/j.dnarep.2004.02.009
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发表时间：
2004-07-02
期刊：
影响因子：
3.8
通讯作者：
Marinus, MG
中科院分区：
文献类型：
--
作者：
Nowosielska, A;Calmann, MA;Marinus, MG
为了测量顺铂（顺式-二氨基二氯铂（II））诱导的重组，我们使用了一种定性染色体内测定法，该测定法利用含有非重叠缺失的重复失活lac操纵子并选择Lac（+）重组体。这两个操纵子被一个Mb分开，其中一个的转化产生Lac（+）表型。自发和顺铂诱导重组的Lac（+）形成需要recA、recBC、ruvA、ruvB、ruvC、priA和polA基因的产物。recF，recO，recR和recJ基因的失活减少顺铂诱导的，但不是自发的，重组。对PriA和RecBC的依赖性表明，在DNA损伤处的复制叉停滞或崩溃以形成双链断裂后诱导重组。缺乏重组诱导的反式DDP表明，重组病变顺铂嘌呤嘌呤链内交联。(C)2004 Elsevier B. V.保留所有权利。
To measure cisplatin (cis-diaminodichloroplatinum(II))-induced recombination, we have used a qualitative intrachromosomal assay utilizing duplicate inactive lac operons containing non-overlapping deletions and selection for Lac(+) recombinants. The two operons are separated by one Mb and conversion of one of them yields the Lac(+) phenotype. Lac(+) formation for both spontaneous and cisplatin-induced recombination requires the products of the recA, recBC, ruvA, ruvB, ruvC, priA and polA genes. Inactivation of the recF, recO, recR and recJ genes decreased cisplatin-induced, but not spontaneous, recombination. The dependence on PriA and RecBC suggests that recombination is induced following stalling or collapse of replication forks at DNA lesions to form double strand breaks. The lack of recombination induction by trans-DDP suggests that the recombinogenic lesions for cisplatin are purine-purine intrastrand crosslinks. (C) 2004 Elsevier B.V. All rights reserved.