High prevalence of HPV 16 in South African women with cancer of the cervix and cervical intraepithelial neoplasia
High prevalence of HPV 16 in South African women with cancer of the cervix and cervical intraepithelial neoplasia
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DOI：
10.1002/jmv.10479
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发表时间：
2003-10-01
影响因子：
12.7
通讯作者：
Williamson, AL
中科院分区：
文献类型：
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作者：
Kay, P;Soeters, R;Williamson, AL
尽管在南非的宫颈癌和宫颈肿瘤的发病率很高，但在该地区进行的研究很少，以确定哪些人乳头瘤病毒（HPV）类型与高级别宫颈上皮内瘤变病变和宫颈癌的发展相关。为了调查这些患病率，从56名宫颈癌妇女和141名组织学诊断为宫颈上皮内瘤样病变2或3个病变的妇女中获得打孔活检。采用套式聚合酶链反应（PCR）检测HPV DNA，并采用限制性片段长度多态性分析进行HPV分型。47例（94%）宫颈癌和114例（88%）宫颈上皮内瘤样病变2/3活检组织中HPV DNA阳性。宫颈癌活检中检出的HPV类型的流行率为HPV 16（82%）、HPV 18（10%）、HPV 33（10%）、HPV 31（2%）、HPV 58（2%）、HPV 35（2%）和HPV 59（2%）。宫颈上皮内瘤样病变中HPV 16型占56.6%，HPV 33型占14%，HPV 31型占10.9%，HPV X型占7%，HPV 52型占3.9%，HPV 58型占3.1%，HPV 35型占2.3%，HPV 18型占1.6%，HPV 11型占0.8%。对未通过RFLP分型的9个片段中的5个（命名为HPV-X）进行测序，得到HPV 6（1/5）、HPV 26（2/5）、HPV 68（1/5）和candHPV 87（1/5）。在一个宫颈癌活组织检查和四个来自宫颈上皮内瘤变3级病变的活组织检查中检测到HPV 58，并显示其为先前描述的变体[威廉姆森和Rybicki（1991）J. Med. Virol. 33：165-171]。此外，2级宫颈上皮内瘤变病变显示携带HAN 2294型HPV（cand HPV 87）。这项研究的结果表明，宫颈癌和宫颈上皮内瘤样病变2/3在很大程度上与这组南非妇女的HPV 16感染有关，因此，有效的HPV 16疫苗应该可以预防来自南非这一地区的大部分妇女患宫颈癌。(C)2003 Wiley-Liss，Inc.
Despite the high prevalence of cervical cancer and cervical neoplasias in South Africa, few studies have been performed in this region to establish which human papillomavirus (HPV) types are associated with the development of high-grade cervical intraepithelial neoplasia lesions and cervical cancer. To investigate these prevalence rates, punch biopsies were obtained from 56 women with cervical cancer and 141 women with histologically diagnosed cervical intraepithelial neoplasia 2 or 3 lesions. Nested polymerase chain reaction (PCR) using consensus degenerate PCR primers was performed for the detection of HPV DNA and HPV typing was done by restriction fragment length polymorphism. Forty-seven (94%) of the cervical cancer and 114 (88%) of the cervical intraepithelial neoplasia 2/3 biopsies were positive for HPV DNA. The prevalence rates of the HPV types detected in the cervical cancer biopsies were HPV 16 (82%), HPV 18, (10%), HPV 33 (10%), HPV 31 (2%), HPV 58 (2%), HPV 35 (2%), and HPV 59 (2%). The cervical intraepithelial neoplasia lesions contained HPV 16 (56.6%), HPV 33 (14%), HPV 31 (10.9%), HPV X (7%), HPV 52 (3.9), HPV 58 (3.1%), HPV 35 (2.3%), HPV 18 (1.6%), HPV 11 (0.8%). Five of the nine fragments that were not typed by the RFLP, designated HPV-X, were sequenced to give HPV6 (1/5), HPV 26 (2/5), HPV 68 (1/5), and candHPV 87 (1/5). HPV 58 was detected in one cervical cancer biopsy and four biopsies from cervical intraepithelial neoplasia grade 3 lesions and was shown to be a previously described variant [Williamson and Rybicki (1991) J. Med. Virol. 33:165-171]. In addition, a cervical intraepithelial neoplasia grade 2 lesion was shown to harbour HPV type HAN2294 (cand HPV 87). The results of this study indicate that cervical cancer and cervical intraepithelial neoplasia 2/3 are largely associated with HPV 16 infection in this group of South African women and, therefore, an effective HPV 16 based vaccine should prevent the development of cervical cancer in a large proportion of women from this region of South Africa. (C) 2003 Wiley-Liss, Inc.