Limited expression of reticulocalbin-1 in lymphatic endothelial cells in lung tumor but not in normal lung.
Limited expression of reticulocalbin-1 in lymphatic endothelial cells in lung tumor but not in normal lung.
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DOI：
10.1016/j.bbrc.2011.01.077
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发表时间：
2011-02
影响因子：
3.1
通讯作者：
Y. Yoshida;T. Yamashita;K. Nagano;S. Imai;H. Nabeshi;T. Yoshikawa;Y. Yoshioka;Y. Abe;H. Kamada;Y. Tsutsumi;S. Tsunoda
中科院分区：
文献类型：
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作者：
Y. Yoshida;T. Yamashita;K. Nagano;S. Imai;H. Nabeshi;T. Yoshikawa;Y. Yoshioka;Y. Abe;H. Kamada;Y. Tsutsumi;S. Tsunoda
肿瘤中的淋巴管内皮细胞（T-LEC）被认为具有不同于非肿瘤组织中的淋巴管内皮细胞（N-LEC）的特征。然而，这两种类型之间的差异尚未在分子水平上得到很好的分析。在这份报告中，我们进行了差异蛋白质组分析的T-LEC和N-LEC模型，通过培养的LEC在肿瘤条件培养基。通过使用组织微阵列对所鉴定的蛋白质进行表达谱分析，发现网织红蛋白-1在临床来源的T-LEC和肺癌细胞中表达，但在N-LEC中不表达。提示网织红蛋白-1可能是了解T-LEC功能和控制淋巴结转移的重要分子。
Lymphatic endothelial cells in tumors (T-LECs) are considered to have different characteristics from LECs in non-tumor tissues (N-LECs). However, differences between the two types have not been well analyzed at molecular level. In this report, we performed differential proteome analysis of T-LEC and N-LEC models prepared by cultivation of LECs in tumor conditioned medium. By expression profiling of identified proteins using tissue microarrays, reticulocalbin-1 was found to be expressed in clinical specimen-derived T-LECs and lung cancer cells but not N-LECs. It is suggested that reticulocalbin-1 may be an important molecule in understanding T-LEC function and control of lymphatic metastasis.