1,1-Diphenyl-2-picrylhydrazyl Radical Scavenging Activity of Bee Products and Their Constituents Determined by ESR
1,1-Diphenyl-2-picrylhydrazyl Radical Scavenging Activity of Bee Products and Their Constituents Determined by ESR
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DOI：
10.1248/bpb.32.1947
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发表时间：
2009-12-01
影响因子：
2
通讯作者：
Hara, Hideaki
中科院分区：
文献类型：
--
作者：
Izuta, Hiroshi;Narahara, Yukimi;Hara, Hideaki
本工作的目的是利用ESR方法研究蜂产品及其成分的抗氧化性。抗氧化活性以清除DPPH自由基为指标。清除DPPH自由基的活性大小依次为：中国红提取物>巴西绿色茶醇提物>巴西绿色茶水提物>蜂花粉醇提物。许多天然化合物包括在巴西绿色植物中，例如咖啡酰奎尼酸衍生物[3，4-二-咖啡酰奎尼酸（3，4-CQA）、3，5-二-咖啡酰奎尼酸（3，5-CQA）和绿原酸（ChA）]和肉桂酸衍生物[阿替匹林C、baccharin、rho-香豆酸和drupanin]。咖啡酰奎尼酸衍生物表现出与抗坏血酸和trolox一样强的DPPH自由基清除活性。在肉桂酸衍生物中，阿替匹林C具有较强的DPPH自由基清除活性。咖啡酸苯乙酯（CAPE）是中国红色素中的一种成分，具有较强的清除DPPH自由基的活性，其作用强于抗坏血酸和曲洛酮。咖啡酰奎尼酸的代谢产物咖啡酸具有较强的DPPH自由基清除活性，而奎尼酸则无此活性。因此，巴西和中国枸杞及其成分（咖啡酰奎宁酸衍生物和CAPE）似乎是DPPH自由基的强大清除剂，其效果可能部分取决于其咖啡酰基的性质。
The aim of this work was to investigate the antioxidant property of honeybee products and their constituents using an ESR method. Antioxidative activity was evaluated as the scavenging activity of 1,1-diphenvi-2-picrylhydrazyl (DPPH) radical. The DPPH radical scavenging activities, in descending order, were: ethanol extract of Chinese red propolis>ethanol extract of Brazilian green propolis>water extract of Brazilian green propolis>ethanol extract of bee pollen. Many natural compounds are included in Brazilian green propolis, such as caffeoylquinic acid derivatives [3,4-di-caffeovlquinic acid (3,4-CQA), 3,5-di-caffeovlquinic acid (3,5-CQA), and chlorogenic acid (ChA)] and cinnamic acid derivatives [artepillin C, baccharin, rho-coumaric acid, and drupanin]. Caffeoylquinic acid derivatives exhibited DPPH radical scavenging activity as strong as that of ascorbic acid and trolox. Among the cinnamic acid derivatives, artepillin C exhibited relatively strong DPPH radical scavenging activity. Caffeic acid phenethyl ester (CAPE), a constituent of Chinese red propolis, exhibited potent DPPH radical scavenging activity, stronger than that of ascorbic acid and trolox. Caffeic acid, a metabolite of caffeoylquinic acid, exhibited powerful DPPH radical scavenging activity, while quinic acid, another metabolite of caffeoylquinic acid, had no such activity. Both Brazilian and Chinese propolis and their constituents (caffeoylquinic acid derivatives and CAPE) therefore appear to be powerful scavengers of DPPH radical, and the effects may be partly dependent on the nature of their caffeoyl groups.