In vivo microdialysis sampling of cytokines from rat hippocampus: comparison of cannula implantation procedures.
In vivo microdialysis sampling of cytokines from rat hippocampus: comparison of cannula implantation procedures.
复制标题
大鼠海马细胞因子的体内微透析取样：插管植入程序的比较。
DOI：
10.1021/cn400025m
复制
发表时间：
2013
影响因子：
5
通讯作者：
Stenken,JulieA
中科院分区：
文献类型：
--
作者：
Vasicek,ThaddeusW;Jackson,MatthewR;Poseno,TinaM;Stenken,JulieA
细胞因子是在免疫学领域中非常重要的信号蛋白，因为这些蛋白影响免疫系统中的不同细胞。除了它们在免疫系统中的重要性外，这些蛋白质最近被称为中枢神经系统内的第三个化学通信网络。在机械损伤后，细胞因子在协调组织内的免疫反应中所起的作用会导致潜在的并发症，如果细胞因子的来源(即，创伤与疾病)是重要的。微渗析采样在收集中枢神经系统内许多不同的溶质方面得到了广泛的应用。然而，植入微透析引导导管和探头会造成组织损伤。在这项研究中，我们比较了4 mm，100 kDa截留分子量(MWCO)聚醚砜膜微透析探针植入雄性SD大鼠海马区后，透析液中细胞因子水平的差异。分别于插管后即刻(第0天)、插管后第7天(第7天)、第0天和第7天重复取血，采用多重微珠免疫分析法检测透析液中CCL2(MCP-1)、CCL3(MIP-1α)、CCL5(RANTES)、CXCL1(KC/GRO)、CXCL2(MIP-2)、IL-1β、IL-6和IL-10。不同治疗组之间细胞因子浓度的差异被观察到，在第7天插管动物中测量到更高水平的炎性细胞因子。只有CCl3(MIP-1α)、CXCl1(KC/GRO)、CXCl2(MIP-2)和IL-10超过了大多数透析液的检测限值，它们的浓度通常在每毫升低到高(10-1000)微微克范围内。本文描述的工作为以啮齿动物中枢神经系统细胞因子的微透析取样进行其他基础研究奠定了基础。
Cytokines are signaling proteins that have been of significant importance in the field of immunology, since these proteins affect different cells in the immune system. In addition to their immune system significance, these proteins have recently been referred to as a third chemical communication network within the CNS. The role that cytokines play in orchestrating the immune response within tissues after a mechanical injury leads to potential complications if the source of cytokines (i.e., trauma vs disease) is of interest. Microdialysis sampling has seen wide use in collection of many different solutes within the CNS. Yet, implantation of microdialysis guide cannulas and the probes creates tissue injury. In this study, we compared the differences in cytokine levels in dialysates from 4 mm, 100 kDa molecular weight cutoff (MWCO) polyethersulfone membrane microdialysis probes implanted in the hippocampus of male Sprague–Dawley rats. Comparisons were made between animals that were dialyzed immediately after cannula implantation (day 0), 7 days post cannula implantation (day 7), and repeatedly sampled on day 0 and day 7. Multiplexed bead-based immunoassays were used to quantify CCL2 (MCP-1), CCL3 (MIP-1α), CCL5 (RANTES), CXCL1 (KC/GRO), CXCL2 (MIP-2), IL-1β, IL-6, and IL-10 in dialysates. Differences in cytokine concentrations between the different treatment groups were observed with higher levels of inflammatory cytokines measured in day 7 cannulated animals. Only CCL3 (MIP-1α), CXCL1 (KC/GRO), CXCL2 (MIP-2), and IL-10 were measured above the assay limits of detection for a majority of the dialysates, and their concentrations were typically in the low to high (10–1000) picogram per milliliter range. The work described here lays the groundwork for additional basic research studies with microdialysis sampling of cytokines in rodent CNS.