Protection of rainbow trout from infectious hematopoietic necrosis (IHN) by injection of infectious pancreatic necrosis virus (IPNV) or Poly(I:C)
Protection of rainbow trout from infectious hematopoietic necrosis (IHN) by injection of infectious pancreatic necrosis virus (IPNV) or Poly(I:C)
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DOI：
10.3354/dao02000
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发表时间：
2009-02-12
影响因子：
1.4
通讯作者：
Yoshimizu, Mamoru
中科院分区：
文献类型：
--
作者：
Kim, Hyoung Jun;Oseko, Norihisa;Yoshimizu, Mamoru
最近有报道，预防对传染性造血细胞坏死病毒（IHNV）在鱼类诱导预先暴露于传染性胰腺坏死病毒（IPNV）。在这里，建立IHNV免疫虹鳟鱼虹鳟鱼IHNV攻击后，非致死性预感染IPNV。此外，合成的双链RNA聚肌苷酸聚胞苷酸，聚（I：C），干扰素（IFN）的诱导剂，作为替代IPNV诱导的非特异性抗病毒状态和随后的IHNV特异性免疫力的鱼进行了评估。IPNV预感染虹鳟鱼7 d后可免于IHNV攻击（相对存活率，RPS：68.8%），并且在存活者的血清中检测到IHNV特异性抗体。此外，这些存活的鱼表现出91.6%，RPS时，再用IHNV攻击后28天的主要IHNV攻击。因此，鱼似乎通过预先注射IPNV后的IHNV攻击获得IHNV特异性免疫。用Poly（I：C）预注射的鱼在2天后也高度保护免于IHNV攻击（RPS：95.2%），并且在存活者中也检测到IHNV特异性抗体。存活者在初次IHNV攻击后21和49天用IHNV再攻击后显示100%的存活率。因此，虹鳟鱼中的IHNV免疫是通过在预先注射IPNV或Poly（I：C）后用活IHNV攻击诱导的。使用Poly（I：C）诱导抗病毒状态，保护虹鳟鱼免受IHNV的致死疫苗接种剂量的影响，可能适用于更广泛的鱼类物种和鱼类致病性病毒。
It was recently reported that prophylaxis against infectious hematopoietic necrosis virus (IHNV) in fish was induced by pre-exposure to the infectious pancreatic necrosis virus (IPNV). Here the establishment of IHNV immunity in rainbow trout Oncorhynchus mykiss was investigated by IHNV challenge following non-lethal pre-infection with IPNV. Also, synthetic double-stranded RNA polyinosinic polycytidylic acid, Poly(I:C), an inducer for interferon (IFN), was evaluated as a substitute for IPNV induction of the non-specific antiviral state and subsequent IHNV-specific immunity in fish. Rainbow trout pre-infected with IPNV were protected from IHNV challenge 7 d later (relative percentage survival, RPS: 68.8%), and IHNV-specific antibodies were detected in sera from the survivors. Moreover, these surviving fish showed 91.6%, RPS when re-challenged with IHNV 28 d after the primary IHNV challenge. Thus, fish appear to acquire IHNV-specific immunity through the IHNV challenge following pre-injection with IPNV. Fish pre-injected with Poly(I:C) were also highly protected from IHNV challenge 2 d later (RPS: 95.2 %), and IHNV-specific antibodies were also detected amongst survivors. The Survivors showed a 100% survival rate following re-challenge with IHNV both 21 and 49 d after the primary IHNV challenge. Thus, IHNV immunity in rainbow trout is induced by challenge with live IHNV following pre-injection with either IPNV or Poly(I:C). The use of Poly(I:C) to induce an anti-viral state protecting rainbow trout from an otherwise lethal vaccination dose of IHNV may have application to a wider range of fish species and fish pathogenic viruses.