Growth performance and nutrient digestibility in pigs fed wheat distillers dried grains with solubles-based diets supplemented with a multicarbohydrase enzyme
Growth performance and nutrient digestibility in pigs fed wheat distillers dried grains with solubles-based diets supplemented with a multicarbohydrase enzyme
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DOI：
10.2527/jas.2008-1195
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发表时间：
2009-07-01
影响因子：
3.3
通讯作者：
Nyachoti, C. M.
中科院分区：
文献类型：
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作者：
Emiola, I. A.;Opapeju, F. O.;Nyachoti, C. M.
本试验旨在研究在小麦酒糟（DDGS）基础日粮中添加复合糖酶对生长肥育猪生产性能和养分消化率的影响。在Exp.根据性别和BW，将48头BW为36.5 +/- 1.03 kg（平均值+/- SD）的猪随机分配到24个围栏中（每围栏2头猪），并将猪随机分配到实验饮食中（每种饮食6个围栏）。饮食处理是阳性对照（PC）和阴性对照（NC），阳性对照（PC）配制为满足或超过NRC（1998）的营养需求，阴性对照（NC）的DE和赖氨酸分别从PC饮食中减少4%和5%，并且不含或含2个水平的酶混合物。水平1酶提供2，600 U木聚糖酶，1，200 U β-葡聚糖酶和1，300 U纤维素酶/kg日粮;水平2酶提供水平1中每种酶活性的两倍/kg日粮。氧化铬被用作难消化的标记物。NC组日增重显著低于PC组（P < 0.05）。添加复合酶制剂可显著提高日增重（P < 0.01）。ADFI不受饮食治疗的影响。PC饮食有更大的（P = 0.04）G：F与NC饮食相比，但不是添加酶的饮食。与NC组相比，PC组对N、GE和乙醚提取物的消化率显著提高（P < 0.01）。在NC日粮中添加2级酶可显著提高（P < 0.05）DM、N、GE和乙醚提取物的消化率，使其达到与PC日粮相似的水平（P > 0.05）。在Exp.将2，4头回肠插管公猪（80.8 kg BW; SD = 0.65）以4 × 4拉丁方设计分配至4种饲料中，2 × 2析因处理安排。试验日粮中含有15或30%的小麦DDGS，不加酶或加酶饲喂。酶补充剂每kg日粮提供2，200 U木聚糖酶、1，100 U β-葡聚糖酶和1，200 U纤维素酶。添加酶制剂的日粮中，干物质、有机物和能量的回肠表观消化率（AID）显著高于未添加酶制剂的日粮（P < 0.05）。小麦DDGS添加量与酶制剂对DM和Ser的AID存在交互作用（P < 0.05），在小麦DDGS添加量为30%的日粮中，添加酶制剂对DM和Ser的AID有显著提高（P < 0.05），但对15%小麦DDGS的AID无显著影响。结果表明，在30%小麦DDGS基础日粮中添加多酶制剂，可提高生长猪的生产性能和DM、N、GE、粗纤维的表观全消化道消化率，提高肥育猪的营养物质利用率。
The objective was to investigate the effect of supplementing a wheat distillers dried grains with solubles (wheat DDGS)-based diet with carbohydrase enzyme blends on growth performance and nutrient digestibilities in growing and finishing pigs. In Exp. 1, 48 pigs with a BW of 36.5 +/- 1.03 kg (mean +/- SD) were randomly assigned to 24 pens (2 pigs per pen) on the basis of sex and BW, and pigs were randomly allocated to experimental diets (6 pens per diet). Dietary treatments were a positive control (PC) formulated to meet or exceed NRC (1998) nutrient requirements and a negative control (NC) with DE and Lys reduced by 4 and 5%, respectively, from the PC diet and fed without or with an enzyme blend at 2 levels. Level 1 enzyme supplied 2,600 U of xylanase, 1,200 U of beta-glucanase, and 1,300 U of cellulase per kg of diet; level 2 enzyme provided twice the activities for each enzyme in level 1 per kilogram of diet. Chromic oxide was used as an indigestible marker. Pigs fed the NC diet had less (P < 0.05) ADG than those fed the PC diet. Supplementing the NC diet with enzyme blends linearly increased ADG (P < 0.01). The ADFI was not affected by dietary treatment. The PC diet had greater (P = 0.04) G: F compared with the NC diet, but not the enzyme-supplemented diets. Pigs fed the PC diet had greater (P < 0.01) digestibilities of N, GE, and ether extract compared with those fed the NC diet. Addition of level 2 enzyme to the NC diet improved (P < 0.05) digestibilities of DM, N, GE, and ether extract to levels similar (P > 0.05) to those fed the PC diet. In Exp. 2, 4 ileal cannulated barrows (80.8 kg of BW; SD = 0.65) were allotted to 4 diets in a 4 x 4 Latin square design with a 2 x 2 factorial arrangement of treatments. Experimental diets contained 15 or 30% wheat DDGS and were fed without or with enzyme addition. The enzyme supplement supplied 2,200 U of xylanase, 1,100 U of beta-glucanase, and 1,200 U of cellulase per kg of diet. Apparent ileal digestibilities (AID) of DM, OM, and energy in enzyme-supplemented diets were greater (P < 0.05) than in unsupplemented diets. There was an interaction effect (P < 0.05) of wheat DDGS inclusion level and enzyme supplementation on AID of DM and Ser. Enzyme supplementation improved (P < 0.05) AID of DM in the diet with 30% wheat DDGS but not 15% wheat DDGS. The results shows that multienzyme supplementation of 30% wheat DDGS-based diet improved growth performance and apparent total tract digestibility of DM, N, GE, and crude fiber in growing pigs and AID of nutrients in finishing pigs.