Characterization of Terpene Synthase from Tea Green Leafhopper Being Involved in Formation of Geraniol in Tea (Camellia sinensis) Leaves and Potential Effect of Geraniol on Insect-Derived Endobacteria
Characterization of Terpene Synthase from Tea Green Leafhopper Being Involved in Formation of Geraniol in Tea (Camellia sinensis) Leaves and Potential Effect of Geraniol on Insect-Derived Endobacteria
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DOI：
10.3390/biom9120808
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发表时间：
2019-11
期刊：
影响因子：
5.5
通讯作者：
Ying Zhou;Xiaoyu Liu;Ziyin Yang
中科院分区：
文献类型：
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作者：
Ying Zhou;Xiaoyu Liu;Ziyin Yang
当昆虫攻击植物时，昆虫衍生的激发子和机械损伤诱导植物挥发物的形成和释放，这些挥发物具有重要的生态功能和风味特性。这些事件主要是在模式植物中研究的，而不是作物植物。研究表明，茶绿色小绿叶蝉（Empoasca（Matsumurasca）onukii Matsuda）对茶树香叶醇合成酶粗酶活性无影响，但对香叶醇合成酶粗酶活性有显著的诱导作用。E.（M.）onukii专门从香叶醇二磷酸生产香叶醇。此外，还从大肠杆菌中分离到一种萜烯合酶（EoTPS）。（M.）onukii。这种萜烯合酶能够在体外将香叶醇二磷酸转化为香叶醇。此外，香叶醇在体外对从大肠杆菌中分离的约氏不动杆菌有抑制作用。（M.）onukii。这说明刺吸式昆虫的诱导子可以诱导作物挥发物的形成，并促进了我们对植物挥发物在作物-昆虫-微生物相互作用中的作用的认识。
When insects attack plants, insect-derived elicitors and mechanical damage induce the formation and emission of plant volatiles that have important ecological functions and flavor properties. These events have mainly been studied in model plants, rather than crop plants. Our study showed that tea green leafhopper (Empoasca (Matsumurasca) onukii Matsuda), a major pest infesting tea attack significantly induced the emission of geraniol from tea leaves, but did not affect the crude enzyme activity of geraniol synthase in tea leaves. An enzyme extract of E. (M.) onukii specifically produced geraniol from geraniol diphosphate. Furthermore, a terpene synthase (EoTPS) was isolated from E. (M.) onukii. This terpene synthase was able to convert geraniol diphosphate to geraniol in vitro. In addition, geraniol had in vitro ability to inhibit the growth of Acinetobacter johnsonii that is endobacterial isolated from E. (M.) onukii. This information illustrates that elicitors from piercing-sucking insects can induce the formation of volatiles from crop plants and advances our understanding of the roles of plant volatiles in the interaction among crops-insects-microorganisms.