Ingestion of guar-gum hydrolysate partially restores calcium absorption in the large intestine lowered by suppression of gastric acid secretion in rats
Ingestion of guar-gum hydrolysate partially restores calcium absorption in the large intestine lowered by suppression of gastric acid secretion in rats
复制标题
DOI：
10.1017/s0007114599000562
复制
发表时间：
1999-04-01
影响因子：
3.6
通讯作者：
Ohta, T
中科院分区：
文献类型：
--
作者：
Hara, H;Suzuki, T;Ohta, T
我们研究了在质子泵抑制剂奥美拉唑抑制胃酸分泌的条件下，饲喂瓜尔胶水解物(GGH)对大鼠小肠和大肠钙吸收的影响。我们还研究了盲肠在影响这些效应中的作用。实验采用2×2×2析因设计，分为两组(含GGH饲料(50g/kg饲料)和不含GGH饲料)、两个注射组(奥美拉唑和赋形剂)和两个手术组(假手术和盲肠切除)。奥美拉唑(30 mg/kg体重/d)连续给药8d，大鼠的表观钙吸收率低于给药组。摄入GGH可部分恢复奥美拉唑降低的钙吸收。然而，这种增加的钙吸收不足以满足需要，因为饲料中的钙水平(30g/kg饲料)是正常大鼠的最低需要。GGH饮食引起的钙吸收的小幅增加被盲肠切除完全消除。饲喂GGH可增加盲肠和结肠内容物中的可溶性钙库，且可溶性钙浓度远高于大肠钙主动转运系统的Kt值或血清钙浓度。这些结果表明，钙的增溶作用不是大肠吸收钙的限制因素。去盲肠大鼠的镁表观吸收率明显低于假手术组，GGH饲喂组的镁表观吸收率高于不喂GGH饲料组，甚至在去盲肠大鼠也是如此。结论：GGH可部分恢复因抑制胃酸分泌而降低的钙吸收，但增加的幅度不足以满足需要。
We examined the effects of feeding guar-gum hydrolysate (GGH), a highly fermentable form of dietary fibre with low viscosity, on Ca absorption in the small and large intestines in rats under conditions in which gastric acid secretion was suppressed by a proton pump inhibitor, omeprazole. We also examined the role of the caecum in influencing these effects. The study was designed in a 2 x 2 x 2 factorial arrangement with two diet (GGH-containing (50 g/kg diet) and GGH-free diets) groups, two injection (omeprazole and vehicle) groups and two operation (sham and caecectomy) groups. Apparent Ca absorption was lower in rats administered omeprazole (30 mg/kg body weight per d) for 8 d than in rats administered the vehicle. ingestion of GGH led to partial restoration of Ca absorption decreased by omeprazole treatment. However, this increment in Ca absorption was not sufficient to meet requirements because the dietary Ca level (30 g/kg diet) was the minimum requirement for the intact rats. The small increment in Ca absorption caused by the GGH diet was completely abolished by caecectomy. Soluble Ca pools in the caecal and colonic contents were increased by feeding GGH, and the soluble Ca concentrations were much higher than the Kt values of the Ca active transport system in the large intestine or the serum Ca concentration. These findings suggest that Ca solubilization is not a limiting factor for Ca absorption in the large intestine. Apparent Mg absorption was clearly lower in caecectomized rats than in sham-operated rats, and higher in the GGH-fed groups than in the groups fed on the GGH-free diet, even in the case of caecectomized rats. We conclude that Ca absorption lowered by inhibition of gastric acid secretion is partially restored in rats fed with GGH, but the increment is not sufficient to meet requirements.