Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging
Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging
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DOI：
10.1021/acschembio.8b00200
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发表时间：
2018-08-01
影响因子：
4
通讯作者：
Nagai, Takeharu
中科院分区：
文献类型：
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作者：
Arai, Yoshiyuki;Takauchi, Hiroki;Nagai, Takeharu
基于单分子定位显微镜（SMLM）的超分辨率成像技术，借助光开关荧光探针和复杂的显微系统，突破了光学显微镜在活体样品中的衍射极限。在这里，我们开发了一种荧光蛋白，SPOON，它可以通过激发光照明关闭，并通过热诱导脱水打开，从而导致明显的自发眨眼行为。SPOON的这种独特特性提供了一个简单的基于SMLM的超分辨率成像平台，只需一个488 nm激光器。
Super-resolution imaging techniques based on single molecule localization microscopy (SMLM) broke the diffraction limit of optical microscopy in living samples with the aid of photoswitchable fluorescent probes and intricate microscopy systems. Here, we developed a fluorescent protein, SPOON, which can be switched off by excitation light illumination and switched on by thermally induced dehydration, resulting in an apparent spontaneous blinking behavior. This unique property of SPOON provides a simple SMLM-based super-resolution imaging platform which requires only a single 488 nm laser.