Three-dimensional reconstruction of metabolic data from quantitative autoradiography of rat brain.
Three-dimensional reconstruction of metabolic data from quantitative autoradiography of rat brain.
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大鼠脑定量放射自显影代谢数据的三维重建。
DOI：
10.1152/ajpendo.1984.247.3.e412
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Hawkins,RA
中科院分区：
文献类型：
--
作者：
Hibbard,LS;Hawkins,RA
定量放射自显影术是一种通过测定血流量、葡萄糖利用率或必需营养素运输来研究脑功能的强有力方法。放射自显影图像包含大量潜在有用的信息，但传统的分析实际上只能在实验者任意选择的少量点上对数据进行采样，以代表离散的大脑结构。为了更充分地利用图像数据，需要计算机方法来获取，存储，定量分析和显示。我们已经开发了一个执行这些任务的计算机程序系统，并具有以下特征：1）使用交互式图形编辑和分析单个图像，2）仅使用图像本身的数学特性将图像彼此配准的自动图像对准算法，3）从不同实验系列图像集中的等效图像计算平均图像，4）差图像的计算（例如，实验减去对照），具有仅显示估计为统计学显著的差异的选项，和5）使用高速计算机图形系统显示三维重建的连续图像代谢图。
Quantitative autoradiography is a powerful method for studying brain function by the determination of blood flow, glucose utilization, or transport of essential nutrients. Autoradiographic images contain vast amounts of potentially useful information, but conventional analyses can practically sample the data at only a small number of points arbitrarily chosen by the experimenter to represent discrete brain structures. To use image data more fully, computer methods for its acquisition, storage, quantitative analysis, and display are required. We have developed a system of computer programs that performs these tasks and has the following features: 1) editing and analysis of single images using interactive graphics, 2) an automatic image alignment algorithm that places images in register with one another using only the mathematical properties of the images themselves, 3) the calculation of mean images from equivalent images in different experimental serial image sets, 4) the calculation of difference images (e.g., experiment-minus-control) with the option to display only differences estimated to be statistically significant, and 5) the display of serial image metabolic maps reconstructed in three dimensions using a high-speed computer graphics system.