Cu-T-1 Sensor for Versatile Analysis
Cu-T-1 Sensor for Versatile Analysis
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用于多功能分析的 Cu-T-1 传感器
DOI：
10.1021/acs.analchem.7b04971
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发表时间：
2018
影响因子：
7.4
通讯作者：
Jiang Xingyu
中科院分区：
文献类型：
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作者：
Dong Mingling;Zheng Wenshu;Chen Yiping;Ran Bei;Qian Zhiyong;Jiang Xingyu
传统的磁传感器通常采用铁基磁性材料作为信号探针。在这项工作中，我们发现Cu（II）也是一个有用的纵向弛豫时间（T1）信号为基础的磁探针。采用浴铜灵二磺酸二钠盐（BCS）螯合Cu（I），在水溶液中形成稳定的Cu（I）-BCS配合物，发现Cu（II）水溶液与Cu（I）-BCS配合物水溶液的水质子T1值有显著差异。通过氧化还原反应将Cu（II）转化为Cu（I），再与BCS络合，导致T1的明显变化，从而可以作为磁信号读出，这是Cu-T1传感器的基础。Cu（II）和Cu（I）之间的许多氧化还原反应使Cu-T1传感器不仅可以实现“一步模式”的测定，如抗坏血酸，蛋白质和碱性磷酸酶，而且还可以实现“多步模式”的免疫测定，如生物大分子和小分子。该Cu-T1传感器采用Cu离子作为信号读出，为生化分析提供了一种替代工具。
Conventional magnetic sensors usually employ Fe-based magnetic materials as signal probes. In this work, we find that Cu(II) is also a useful longitudinal relaxation time (T1) signal-based magnetic probe. We adopt bathocuproinedisulfonic acid disodium salt hydrate (BCS) to chelate Cu(I) and form a stable Cu(I)–BCS complex in aqueous solution and find the significant difference in the T1value of water protons between Cu(II) aqueous solution and Cu(I)–BCS complex aqueous solution. Redox reaction can convert Cu(II) to Cu(I) followed by the complexation of BCS, which results in apparent change of T1that can serve as magnetic signal readout, which is the basis of this Cu-T1sensor. Many redox reactions between Cu(II) and Cu(I) allow this Cu-T1sensor to not only realize “one-step mode” assay such as ascorbic acid, protein, and alkaline phosphatase but also enable “multi-step mode” immunoassay, such as biomacromolecules and small molecules. This Cu-T1sensor employs Cu ion as signal readout, providing an alternative tool for biochemical analysis.