Time-lapse microscopy reveals unique roles for kinesins during anaphase in budding yeast.
Time-lapse microscopy reveals unique roles for kinesins during anaphase in budding yeast.
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DOI：
10.1083/jcb.143.3.687
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发表时间：
1998-11-02
期刊：
影响因子：
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通讯作者：
Murray AW
中科院分区：
文献类型：
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作者：
Straight AF;Sedat JW;Murray AW
有丝分裂纺锤体是一个复杂的动态结构。在芽殖酵母中的遗传分析已经确定了两组驱动蛋白样马达，Cin8p和Kip1p，以及Kar3p和Kip3p，它们在有丝分裂中具有重叠的功能。我们已经研究了三个这些电机的作用，通过视频显微镜的电机突变体的微管和着丝粒标记有绿色荧光蛋白。尽管它们的功能重叠，但每个运动突变体在有丝分裂中都有特定的缺陷：cin8Δ突变体缺乏后期B的快速期，kip 1 Δ突变体在后期B的缓慢期显示出缺陷，而kip 3 Δ突变体延长了后期的持续时间，直到纺锤体变得比细胞更长。kip3Δ和kip1Δ突变体影响后期的持续时间，而cin8Δ不影响。
The mitotic spindle is a complex and dynamic structure. Genetic analysis in budding yeast has identified two sets of kinesin-like motors, Cin8p and Kip1p, and Kar3p and Kip3p, that have overlapping functions in mitosis. We have studied the role of three of these motors by video microscopy of motor mutants whose microtubules and centromeres were marked with green fluorescent protein. Despite their functional overlap, each motor mutant has a specific defect in mitosis: cin8Δ mutants lack the rapid phase of anaphase B, kip1Δ mutants show defects in the slow phase of anaphase B, and kip3Δ mutants prolong the duration of anaphase to the point at which the spindle becomes longer than the cell. The kip3Δ and kip1Δ mutants affect the duration of anaphase, but cin8Δ does not.