Redox Proteomics Identification of Oxidatively Modified Myocardial Proteins in Human Heart Failure: Implications for Protein Function
Redox Proteomics Identification of Oxidatively Modified Myocardial Proteins in Human Heart Failure: Implications for Protein Function
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DOI：
10.1371/journal.pone.0035841
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发表时间：
2012-05-14
期刊：
影响因子：
3.7
通讯作者：
Banfi, Cristina
中科院分区：
文献类型：
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作者：
Brioschi, Maura;Polvani, Gianluca;Banfi, Cristina
心力衰竭时氧化应激的增加可能是心力衰竭（HF）的发病机制之一。本研究的目的是鉴定HF患者心肌中的氧化蛋白质，并分析氧化对蛋白质功能的影响。用免疫分析法检测14例衰竭和13例非衰竭心脏左心室组织中的羰基化蛋白，并进行蛋白质组学鉴定。HL-1心肌细胞在HF相关刺激物存在下孵育，以评估活性氧（ROS）的产生、蛋白质羰基化的诱导及其对蛋白质功能的影响。HF患者的羰基化蛋白水平显著高于对照组（p
Increased oxidative stress in a failing heart may contribute to the pathogenesis of heart failure (HF). The aim of this study was to identify the oxidised proteins in the myocardium of HF patients and analyse the consequences of oxidation on protein function. The carbonylated proteins in left ventricular tissue from failing (n = 14) and non-failing human hearts (n = 13) were measured by immunoassay and identified by proteomics. HL-1 cardiomyocytes were incubated in the presence of stimuli relevant for HF in order to assess the generation of reactive oxygen species (ROS), the induction of protein carbonylation, and its consequences on protein function. The levels of carbonylated proteins were significantly higher in the HF patients than in the controls (p