Bafilomycin A(1) induces apoptosis in PC12 cells independently of intracellular pH
Bafilomycin A(1) induces apoptosis in PC12 cells independently of intracellular pH
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DOI：
10.1016/s0014-5793(96)01182-9
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发表时间：
1996-11-25
期刊：
影响因子：
3.5
通讯作者：
Ohkuma, S
中科院分区：
文献类型：
--
作者：
Kinoshita, K;Waritani, T;Ohkuma, S
用巴弗洛霉素A（1）抑制生长的PC12细胞死亡，显示细胞核中的凋亡染色质凝聚。巴弗洛霉素A（1）诱导的染色质凝聚之前是神经突生长（NOG），需要比NOG更高浓度的巴弗洛霉素A（1），并被放线菌酮和金精三羧酸抑制。NH4Cl（10 mM），另一种亲酸pH干扰剂，既不诱导凋亡染色质浓缩本身，也不抑制巴夫洛霉素A（1）诱导的染色质浓缩，表明巴夫洛霉素A（1）诱导的细胞凋亡在PC12细胞中独立于细胞内pH发生。
PC12 cells growth-arrested with bafilomycin A(1) died showing apoptotic chromatin condensation in the nuclei. The bafilomycin A(1)-induced chromatin condensation was preceded by neurite outgrowth (NOG), required higher concentrations of bafilomycin A(1) than NOG, and was suppressed by cycloheximide and aurintricarboxylic acid. NH4Cl (10 mM), another acidotropic pH perturbing agent, neither induced apoptotic chromatin condensation by itself nor suppressed that induced by bafilomycin A(1), suggesting that bafilomycin A(1)-induced apoptosis occurs independently of intracellular pH in PC12 cells.