S-allylcysteine induces cell cycle arrest and apoptosis in androgen-independent human prostate cancer cells
S-allylcysteine induces cell cycle arrest and apoptosis in androgen-independent human prostate cancer cells
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S-烯丙基半胱氨酸诱导雄激素非依赖性人前列腺癌细胞的细胞周期停滞和细胞凋亡
DOI：
10.3892/mmr.2011.658
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发表时间：
2012-02-01
影响因子：
3.4
通讯作者：
Ye, Zhangqun
中科院分区：
文献类型：
--
作者：
Liu, Zhuo;Li, Mingchao;Ye, Zhangqun
为了增加植物化学补充剂作为化学预防或辅助药物在癌症治疗中的应用，有必要验证其生物学效应及其相关机制。最近，S-烯丙基半胱氨酸（SAC）被鉴定为来自大蒜的有效化合物。本研究的目的是评价SAC对雄激素非依赖性人前列腺癌（PC-3）细胞的抗肿瘤作用，并阐明其可能的机制。将PC-3细胞与三种不同浓度的SAC一起孵育。通过细胞计数试剂盒-8和5-乙炔基-2 '-脱氧尿苷测定法测定细胞生长。流式细胞仪检测细胞周期和细胞凋亡。Western blot检测凋亡相关分子的表达。结果表明，SAC能抑制PC-3细胞的增殖，使细胞周期阻滞于G 0/G1期，并诱导细胞凋亡，同时伴有Bcl-2蛋白表达降低，Bax和caspase 8蛋白表达增加。本研究证明了SAC在体外的化学预防活性，SAC可能是前列腺癌治疗的一个有前途的候选者。
To increase the use of phytochemical supplements as chemoprevention or adjuvant drugs in cancer treatment, it is necessary to verify their biological effects and correlative mechanisms. Recently, S-allylcysteine (SAC) was identified as a potent compound derived from garlic. The aim of this study was to evaluate the anticancer effects of SAC on androgen-independent human prostate cancer (PC-3) cells and to elucidate the possible mechanisms. PC-3 cells were incubated with SAC at three different concentrations. Cell growth was determined by Cell Counting Kit-8 and 5-ethynyl-2'-deoxyuridine assay. Cell cycle and apoptosis were determined by flow cytometric assay. The expression of apoptosis-related molecules was detected by Western blot analysis. We found that SAC suppressed the proliferation of PC-3 cells and led to cell cycle arrest at the G0/G1 phases, as well as inducing cell apoptosis which was accompanied by the decreased expression of Bcl-2 and increased expression of Bax and caspase 8. This study demonstrated the chemopreventive activity of SAC in vitro, and that SAC may be a promising candidate for prostate cancer treatment.