3 DISTINCT MORPHOLOGICAL CLASSES OF RECEPTORS IN FISH OLFACTORY ORGANS
3 DISTINCT MORPHOLOGICAL CLASSES OF RECEPTORS IN FISH OLFACTORY ORGANS
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DOI：
10.1002/cne.902220403
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发表时间：
1984-01-01
影响因子：
2.5
通讯作者：
MARC, RE
中科院分区：
文献类型：
--
作者：
MULLER, JF;MARC, RE
在形态上提出了三类不同的受体神经元：I型纤毛细胞、微绒毛细胞和Tye II纤毛细胞。辣根过氧化物酶[HRP]通过嗅神经轴突逆行运输到金鱼和斑点叉尾鱼的嗅觉器官，证明了这两种鱼的嗅觉器官中都存在这些轴突细胞。用扫描电子显微镜对金鱼嗅觉器官进行了研究，用木瓜酶解离制备了分离细胞，并对酸性磷酸酶进行了超微结构定位。TYE I纤毛细胞与所有脊椎动物的纤毛嗅觉感受器相似。微绒毛细胞存在于大多数鱼类的嗅觉器官和四足犁鼻器中。在金鱼和鲶鱼中，I型纤毛和微绒毛细胞集中在每一层的内侧1/3，最靠近中缝正中。II型纤毛细胞通常被描述为呼吸型或纤毛无感觉细胞。它们在结构上与四足动物鼻腔中的呼吸上皮细胞相似，并具有同步跳动的活动纤毛，表明它们在调节嗅觉上皮细胞上的液体流动方面起到了作用。在金鱼中，它们单独出现，并聚集在整个器官中。在鲶鱼中，它们是从I型纤毛细胞和微绒毛细胞中分离出来的，这些细胞位于每个薄片的外部2/3处。在金鱼和叉尾鱼中，它们的轴突穿过嗅神经到达嗅球；因此，它们是感受性神经元，类似于呼吸道上皮。远端突起为杆状突起的类似受体的细胞被HRP充满，扫描和透射电子显微镜观察。类似结构的细胞在其他地方也有记载，通常被称为杆状细胞，有时被认为是鱼类中的一种单独的受体类型。可见许多杆状突起，纤毛或微绒毛成分部分融合。高水平的酸性磷酸酶活性定位于这些过程，并发现与三种受体类型中的每一种相对应的例子。嗅觉感受器的更替被认为会持续一生。目前的证据支持这一假设，即树突状顶突的融合标志着受体细胞衰老的早期阶段。
Three morphologically distinct classes of receptor-neurons are proposed: type I ciliar cells, microvillar cells and tye II ciliar cells. Retrograde transport of horseradish peroxidase [HRP] by axons in the olfactory nerve to the olfactory organs of goldfish (Carassius auratus) and channel catfish (Ictalurus punctatus) provided evidence that these axon-bearing cells are present in the organs of both species. Goldfish olfactory organs were also stuided with scanning electron microscopy, dissociated with papain for isolated cell preparations and processed for ultrastructural localization of acid phosphatase activity. Tye I ciliar cells are similar to ciliar olfactory receptors found in all vertebrate classes. Microvillar cells are present in the olfactory organs of most fishes and in the tetrapod vomeronasal organ. In goldfish and catfish, type I ciliar and microvillar cells are concentrated on the inner third of each lamella, nearest to the median raphe. Type II ciliar cells have often been described as respiratory-type or ciliated nonsensory cells. They are structurally similar to respiratory epithelial cells in the nasal cavities of tetrapods and have motile cilia that beat synchronously, indicative of their role in mediating fluid flow over the olfactory epithelium. In goldfish they occur singly and in aggregates throughout the organ. In catfish they are segregated from type I ciliar and microvillar cells on the outer 2/3 of each lamella. In goldfish and catfish they have axons that pass through the olfactory nerve to the olfactory bulb; hence, they are receptor neurons as well as analogous to respiratory epithelium. Cells resembling receptors with rod-like distal processes were seen filled with HRP and observed with scanning and transmission electron microscopy. Cells of similar structure were documented elsewhere, often called rod cells, and sometimes considered a separate receptor type in fishes. A number of rod-like processes were found with their ciliar or microvillar components partially fused. High levels of acid phosphatase activity were localized to these processes, and examples were found that corresponded to each of the 3 receptor types. Olfactory receptor turnover is believed to persist through life. The evidence presented supports the hypothesis that fusion of their dendritic apical processes marks an early stage of receptor cell senescence.