ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize
ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize
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ZmMs30 编码新型 GDSL 脂肪酶对于雄性生育力至关重要，并且对于玉米杂交育种很有价值
DOI：
10.1016/j.molp.2019.01.011
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发表时间：
2019-03-04
期刊：
影响因子：
27.5
通讯作者：
Wan, Xiangyuan
中科院分区：
文献类型：
--
作者：
An, Xueli;Dong, Zhenying;Wan, Xiangyuan
基因雄性不育是杂种优势利用和杂种制种的重要手段。虽然已经在植物中发现了大量的GMS基因，但对GDSL脂肪酶成员在花药和花粉发育中的作用知之甚少。在这里，我们报道了一个玉米GMS基因，ZmMs30，它编码一种具有分散催化残基的新型GDSL脂肪酶。酶动力学和活性分析表明，ZmMs30具有脂肪酶活性，并倾向于短碳链底物。ZmMs30在7-9期的玉米花药中特异表达。ZmMs30功能缺失导致花药角质层缺陷，花粉外壁足层不规则，雄性完全不育。细胞学和脂质组学分析表明，ZmMs30对花粉外壁形成和花药角质层发育所需的脂肪代谢途径至关重要。此外，我们发现ZmMs30功能缺失导致的雄性不育在不同遗传背景的自交系中是稳定的，对玉米杂种优势和产量没有任何负面影响，表明ZmMs30在杂交育种和杂交种生产中具有一定的应用价值。然后，我们利用ZmMs30及其突变系开发了一种新的多控制不育系统，并证明了产生理想的GMS系的可行性和杂交玉米种子生产的价值。本研究为揭示玉米花药和花粉发育机制提供了新的思路，为玉米杂交育种提供了有价值的雄性不育系统。
Genic male sterility (GMS) is very useful for hybrid vigor utilization and hybrid seed production. Although a large number of GMS genes have been identified in plants, little is known about the roles of GDSL lipase members in anther and pollen development. Here, we report a maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain. ZmMs30 is specifically expressed in maize anthers during stages 7-9. Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility. Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development. Furthermore, we found that male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn't show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for crossbreeding and hybrid seed production. We then developed a new multi-control sterility system using ZmMs30 and its mutant line, and demonstrated it is feasible for generating desirable GMS lines and valuable for hybrid maize seed production. Taken together, our study sheds new light on the mechanisms of anther and pollen development, and provides a valuable male-sterility system for hybrid breeding maize.