Application of Time-resolved Fluroimmunoassay for Determination of Furaltadone Metabolite 3-Amino-5-morpholinomethyl-2-oxazolidinone
Application of Time-resolved Fluroimmunoassay for Determination of Furaltadone Metabolite 3-Amino-5-morpholinomethyl-2-oxazolidinone
复制标题
应用时间分辨荧光免疫分析法测定呋喃他酮代谢物3-氨基-5-吗啉甲基-2-恶唑烷酮
DOI：
10.1016/s1872-2040(16)60951-9
复制
发表时间：
2016-08-01
影响因子：
1.2
通讯作者：
Shen Yu-Dong
中科院分区：
文献类型：
--
作者：
Deng Li-Hua;Dai Jin-Bo;Shen Yu-Dong
为检测鱼类样品中呋喃他酮代谢产物3-氨基-5-吗啉甲基-2-恶唑烷酮(AMOZ)，建立了Eu~(3+)标记时间分辨荧光免疫分析方法。优化的实验条件为：AMOZA-OVA浓度为0.25ug·mL~(-1)，抗体稀释50000倍，竞争反应时间为50min。在最佳条件下，该方法的检出限为0.01ngmL(-1)，IC_(50)为0.26ngmL(-1)，线性范围(IC20~IC80)为0.025~2.83ngmL(-1)。在3个添加水平下，鱼中Amoz的加标回收率为78.0%~86.0%，相对标准偏差小于15%。IC-TRFIA与高效液相色谱-串联质谱仪对添加食品样品的分析结果具有良好的相关性。所建立的IC-TRFIA方法适用于食品样品中氨甲肼残留量的测定。
To detect furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in fish sample, a Eu3+ labeling time-resolved fluoroimmunoassay (TRFIA) was developed. The experimental conditions were optimized as follows: the AMOZA-OVA concentration was 0.25 mu g mL(-1); the antibody was diluted by 50000 folds, and the competitive reaction time was 50 min. Under the optimal conditions, the method showed a detection limit of 0.01 ng mL(-1), an IC50 of 0.26 ng mL(-1) and a linear range (IC20-IC80) of 0.025-2.83 ng mL(-1). The spiked recoveries of AMOZ in fish at three spiked levels ranged from 78.0% to 86.0%, and the relative standard deviations were less than 15%. Good correlation between ic-TRFIA and high performance liquid chromatography-tandem mass spectrometry was obtained for spiked food samples. The proposed ic-TRFIA method was suitable for the determination of AMOZ residue in food samples.