A 1-Mb resolution radiation hybrid map of the canine genome
A 1-Mb resolution radiation hybrid map of the canine genome
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DOI：
10.1073/pnas.0831002100
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发表时间：
2003-04-29
影响因子：
11.1
通讯作者：
Ostrander, EA
中科院分区：
文献类型：
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作者：
Guyon, R;Lorentzen, TD;Ostrander, EA
纯种犬群体由300个部分近亲繁殖的遗传分离株或品种组成。品种间基因流动的限制，以及对性状的强大选择，导致了剖析哺乳动物简单和复杂性状的遗传基础的独特资源的建立。为此，我们提出了一份全面的犬基因组辐射杂交图谱，该图谱由3,270个标记组成，其中包括1,596个基于微卫星的标记，900个克隆的基因序列和EST，668个犬特异性细菌人工染色体(BAC)末端，以及106个序列标记位点。用RHDF5000-2全基因组辐射杂交板构建MAP，用MultiMAP和TSP/Concorde进行计算。3,270个标记映射到3,021个唯一位置，并定义对应于1Mb的平均标记间距离。我们还定义了一个由325个信息丰富、间隔良好的标记组成的最小筛选集，用于启动全基因组扫描。狗和人类基因组之间明确的同步性，部分作为这项工作的功能，通过鉴定85个保守片段，将允许通过从人类基因组序列中选择候选基因来跟踪连锁的初步发现。这项工作继续将犬类系统定义为寻找导致哺乳动物变异和疾病的基因的选择方法。
The purebred dog population consists of >300 partially inbred genetic isolates or breeds. Restriction of gene flow between breeds, together with strong selection for traits, has led to the establishment of a unique resource for dissecting the genetic basis of simple and complex mammalian traits. Toward this end, we present a comprehensive radiation hybrid map of the canine genome composed of 3,270 markers including 1,596 microsatellite-based markers, 900 cloned gene sequences and ESTs, 668 canine-specific bacterial artificial chromosome (BAC) ends, and 106 sequence-tagged sites. The map was constructed by using the RHDF5000-2 whole-genome radiation hybrid panel and computed by using MULTIMAP and TSP/CONCORDE. The 3,270 markers map to 3,021 unique positions and define an average intermarker distance corresponding to 1 Mb. We also define a minimal screening set of 325 highly informative well spaced markers, to be used in the initiation of genome-wide scans. The well defined synteny between the dog and human genomes, established in part as a function of this work by the identification of 85 conserved fragments, will allow follow-up of initial findings of linkage by selection of candidate genes from the human genome sequence. This work continues to define the canine system as the method of choice in the pursuit of the genes causing mammalian variation and disease.