Development of the larval lymphatic system in zebrafish
Development of the larval lymphatic system in zebrafish
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DOI：
10.1242/dev.145755
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发表时间：
2017-06-01
期刊：
影响因子：
4.6
通讯作者：
Weinstein, Brant M.
中科院分区：
文献类型：
--
作者：
Jung, Hyun Min;Castranova, Daniel;Weinstein, Brant M.
淋巴管系统是一个分层组织的复杂网络，对人体组织液稳态、免疫运输和膳食脂肪吸收至关重要。尽管其重要性，淋巴网络的组装仍然没有完全理解。斑马鱼是一种功能强大的模式生物，可以使用高分辨率成像和复杂的遗传和实验操作来研究淋巴管发育。虽然一些研究已经描述了早期淋巴管的发展在鱼，淋巴管的发展在后期阶段尚未完全阐明。在这项研究中，我们产生了一个新的Tg（mrc1a：egfp）（y251）转基因斑马鱼，使用甘露糖受体，C型1（mrc1a）启动子驱动强大的EGFP表达在淋巴管在所有发展阶段和成年斑马鱼。我们用这条线来描述主干淋巴管网的主要血管的组装，包括发育较晚的主侧支、脊髓、浅外侧和浅节段间支。我们的研究结果表明，主要的主干淋巴管是保守的斑马鱼，并提供了一个全面和完整的描述主干淋巴管组装。
The lymphatic vascular system is a hierarchically organized complex network essential for tissue fluid homeostasis, immune trafficking and absorption of dietary fats in the human body. Despite its importance, the assembly of the lymphatic network is still not fully understood. The zebrafish is a powerful model organism that enables study of lymphatic vessel development using high-resolution imaging and sophisticated genetic and experimental manipulation. Although several studies have described early lymphatic development in the fish, lymphatic development at later stages has not been completely elucidated. In this study, we generated a new Tg(mrc1a:egfp)(y251) transgenic zebrafish that uses a mannose receptor, C type 1 (mrc1a) promoter to drive strong EGFP expression in lymphatic vessels at all stages of development and in adult zebrafish. We used this line to describe the assembly of the major vessels of the trunk lymphatic vascular network, including the later-developing collateral cardinal, spinal, superficial lateral and superficial intersegmental lymphatics. Our results show that major trunk lymphatic vessels are conserved in the zebrafish, and provide a thorough and complete description of trunk lymphatic vessel assembly.